中国中医药信息杂志2024，Vol.31Issue(1)：117-121,5.DOI:10.19879/j.cnki.1005-5304.202211468
健脾益肠散对溃疡性结肠炎大鼠NLRP3信号通路IL-1β、IL-18表达的影响
Effects of Jianpi Yichang Powder on Expressions of IL-1β and IL-18 of NLRP3 Signaling Pathway in Ulcerative Colitis Rats
摘要
Abstract
Objective To explore the effects of Jianpi Yichang Powder on the expressions of interleukin(IL)-1β and IL-18 of NLRP3 signaling pathway in ulcerative colitis(UC)model rats.Methods Ten rats were randomly selected from 40 SD rats as the normal group,and the other rats freely drank 5%dextran sulfate solution for 7 days to replicate UC rats model.The model rats were randomly divided into model group,sulfasalazine group and Jianpi Yichang Powder group,with 10 rats in each group.Jianpi Yichang Powder group and sulfasalazine group were given corresponding liquid medicine for gavage,and the normal and model groups were given equivalent volume distilled water for gavage for consecutive 14 d.The general status was observed,and the disease activity index(DAI)was scored,the contents of NLRP3,apoptosis-associated spotted proteins(ASC),and Caspase-1 in serum were detected by ELISA,the expressions of IL-1β and IL-18 protein and mRNA in colon tissue were detected by immunohistochemistry,Western blot and RT-PCR respectively.Results Compared with the normal group,the general status of the rats in model group was relatively worse,and DAI score significantly increased(P<0.01),the contents of NLRP3,ASC and Caspase-l in serum were significantly increased(P<0.01),the expressions of IL-1β and IL-18 protein and mRNA in colon tissue were significantly increased(P<0.01).Compared with the model group,the general status of the rats in Jianpi Yichang Powder group and sulfasalazine group were significantly improved,DAI score significantly decreased(P<0.01),the contents of NLRP3,ASC and Caspase-l in serum significantly reduced(P<0.05,P<0.01),and the expressions of IL-1β and IL-18 protein and mRNA in colon tissue significantly decreased(P<0.05,P<0.01).Conclusion Jianpi Yichang Powder can inhibit IL-1β and IL-18 expression of NLRP3 signaling pathway to reduce colon immune inflammatory damage,thus play a role in treating UC.关键词
健脾益肠散/溃疡性结肠炎/NLRP3信号通路/白细胞介素-1β/白细胞介素-18/大鼠Key words
Jianpi Yichang Powder/ulcerative colitis/NLRP3 signaling pathway/IL-1β/IL-18/rats分类
医药卫生引用本文复制引用
蔺晓源,李开楊,管洁,刘杰民..健脾益肠散对溃疡性结肠炎大鼠NLRP3信号通路IL-1β、IL-18表达的影响[J].中国中医药信息杂志,2024,31(1):117-121,5.基金项目
国家自然科学基金(82060850) （82060850）
贵州省高层次创新型人才项目(2020年) （2020年）
