中国医药科学2023，Vol.13Issue(23)：17-20,4.DOI:10.20116/j.issn2095-0616.2023.23.04
二甲双胍通过AMPK/Sirt1信号通路延缓心肌细胞衰老
Metformin delays myocardial cell senescence through AMPK/Sirt1 signaling pathway
摘要
Abstract
Objective To explore the effect of metformin on paraquat-induced myocardial cell senescence and its possible mechanism.Methods The model of paraquat-induced myocardial cell senescence was established.The levels of senescence-associated β-galactosidase(SA-β-Gal)staining positive rate and reactive oxygen species(ROS)were used to evaluate the effect of metformin on aging cardiomyocytes,and RNA interference was used to inhibit the expression of the adenosine 5'-monophosphate-activated protein kinase(AMPK),detect the changes of aging-related indicators and AMPK,sirtuin1(Sirt1)protein content,and further explore the possible mechanism of metformin treatment effect.Results After paraquat intervention,the positive rate of cell senescence increased,the expression of ROS increased,and the expressions of AMPK and Sirt1 decreased significantly,with statistically significant differences(P<0.05).Compared with the paraquat group,the proportion of stained positive cells of SA-β-Gal and the expression of ROS levels significantly decreased in the 0.1 mmol/L metformin intervention group,while the expressions of AMPK and Sirt1 proteins increased,with statistically significant differences(P<0.05).Compared with the metformin intervention group,the expression of Sirt1 in the metformin combined with AMPK siRNA intervention group was reduced,and the proportion of stained positive cells of SA-β-Gal and ROS levels increased,with statistically significant differences(P<0.05).Conclusion Metformin can delay the myocardial cell senescence induced by the paraquat,and the mechanism of its protective effect may be related to the upregulation of AMPK and the increase of Sirt1 expression.关键词
二甲双胍/细胞衰老/腺苷酸活化蛋白激酶/沉默信息调节剂2相关酶1Key words
Metformin/Cell senescence/Adenosine monophosphate-activated protein kinase/Sirtuin1分类
医药卫生引用本文复制引用
余星,刘文炽,肖婧雯,张彦..二甲双胍通过AMPK/Sirt1信号通路延缓心肌细胞衰老[J].中国医药科学,2023,13(23):17-20,4.基金项目
福州市科技计划项目(2021-S-187). （2021-S-187）
