中国中医药信息杂志2024，Vol.31Issue(1)：122-127,6.DOI:10.19879/j.cnki.1005-5304.202303277
基于UPLC-MS/MS与G1-熵权法多指标综合评分优选微乐方提取工艺
Optimization of Extraction Process of Weile Prescription Based on Multi-index Comprehensive Evaluation of UPLC-MS/MS and G1-entropy Weight Method
摘要
Abstract
Objective To establish the method for simultaneous determination of six index components in the water extract of Weile Prescription;To optimize the water extraction process.Methods UPLC-MS/MS was used with Waters CORTECS C18 column(2.1 mm×100 mm,1.6 μm)as the chromatographic conditions;the mobile phase was 0.1%formic acid water-acetonitrile with gradient elution;the flow rate was 0.25 mL/min;the column temperature was 40℃;the sample volume was 2 μL.Electrospray negative ion source,positive and negative ion switching multi-reaction monitoring(MRM)mode were detected.Taking the content of six index components(gallic acid,vitexin,paeoniflorin,naringin,hesperidin and glycyrrhizic acid)and extraction rate as evaluation indexes,the weight coefficient of each index was determined by G1-entropy weight method,and the optimum parameters of extraction process were determined by orthogonal experiment design with the amount of water,extraction time and extraction times as investigation factors.Results There was a good linear relationship of the six components in the water extract of Weile Prescription in the concentration range(r>0.999),and the average recovery rate was 96.83%-102.56%,RSD<4.0%.The best technological parameters were as follows:Chinese decoction pieces were soaked in 12 times of water for 2 h,and extracted twice,each time for 1.5 h.Conclusion The UPLC-MS/MS method established in the study for simultaneous determination of six components in Weile Prescription is rapid,simple and sensitive,and the optimized extraction process is stable and feasible,which provides experimental basis for the development and research of the preparation.关键词
超高效液相色谱串联质谱法/G1-熵权法/多指标/提取工艺/正交试验/微乐方Key words
UPLC-MS/MS/G1-entropy weight method/multi-index/extraction process/orthogonal experiment design/Weile Prescription分类
医药卫生引用本文复制引用
李玲慧,许文,李丹,林娟,黄汉明,潘鸿贞..基于UPLC-MS/MS与G1-熵权法多指标综合评分优选微乐方提取工艺[J].中国中医药信息杂志,2024,31(1):122-127,6.基金项目
福建省临床重点专科建设项目-中药临床药学(2022年) （2022年）
福州市卫生健康系统科技创新平台-福州市中医药传承创新平台(2021-S-wp3) （2021-S-wp3）
全国中药特色技术传承人才培训项目(2023年) （2023年）
