中国医药科学2023，Vol.13Issue(23)：21-24,81,5.DOI:10.20116/j.issn2095-0616.2023.23.05
胸腺肽α1对铜绿假单胞菌感染支气管扩张症大鼠的免疫调节作用
Immunomodulatory effect of thymosin-α1 on rats with bronchiectasis infected by pseudomonas aeruginosa
摘要
Abstract
Objective To analyze the immunomodulatory effect of thymosin-α1 on rats with bronchiectasis infected by pseudomonas aeruginosa.Methods A total of 50 Balb/c rats mediated by pseudomonas aeruginosa purchased by our research team from October 2022 to February 2023 were included in the study,and 10 rats were randomly selected for histopathological examination according to routine procedures,all of which were successfully modeled.20 rats receiving a subcutaneous injection of thymosin-α1 were included in the observation group.Another 20 rats receiving a subcutaneous injection of normal saline were included in the control group.Bronchoalveolar lavage fluid(BALF)and peripheral blood were collected from the two groups of rats as test samples,and mitochondrial DNA(mtDNA)content was detected by reverse transcription-polymerase chain reaction(RT-PCR).Immunoblotting assay was used to determine Toll-like receptors(TLRs)9.Helper T cell(Th)17 cell count,regulatory T cell(Treg)cell count,Cluster of Differentiation(CD)4+,and CD8+ cell count were measured by flow cytometry.The levels of interleukin(IL)-2,IL-6,IL-10,and IL-17 were measured using enzyme-linked immunosorbent assay.By virtue of the above-mentioned methods,a comprehensive evaluation of immunomodulation of thymosin-α1 on bronchiectasis infected by pseudomonas aeruginosa was conducted.Results The CD4+ cell count,CD8+ cell count,mtDNA level,Th17 level,and IL-10 level in the observation group were higher than those in the control group,with statistically significant differences(P<0.05).The levels of TLR9,Treg,IL-2,IL-6,and IL-17 in the observation group were lower than those in the control group,with statistically significant differences(P<0.05).Conclusion The immunity of rats with bronchiectasis infected by pseudomonas aeruginosa is low,and thymosin-α1 can improve the condition of rats by regulating their immune functions.关键词
胸腺肽α1/铜绿假单胞菌/支气管扩张症/免疫失衡Key words
Thymosin-α1/Pseudomonas aeruginosa/Bronchiectasis/Immune imbalance分类
医药卫生引用本文复制引用
梁秋亭,谢衬梨,王惠嫦,柳元斌,陈立冲,袁炜良..胸腺肽α1对铜绿假单胞菌感染支气管扩张症大鼠的免疫调节作用[J].中国医药科学,2023,13(23):21-24,81,5.基金项目
广东省医学科学技术研究基金项目(A2021118). （A2021118）
