中国中医药信息杂志2024，Vol.31Issue(1)：128-134,7.DOI:10.19879/j.cnki.1005-5304.202307176
基于UPLC指纹图谱结合化学计量学的经典名方苓桂术甘汤质量评价研究
Study on Quality Evaluation of Classical Formula-Linggui Zhugan Decoction Based on UPLC Fingerprint Chromatogram Combined with Chemometric Analysis
摘要
Abstract
Objective To establish the UPLC fingerprint chromatogram combined with chemometric analysis for the quality evaluation of classical formula Linggui Zhugan Decoction.Methods SHIMADZU Shim-Pack GIST C18 column(100 mm×2.1 mm,2.0 μm)was used with acetonitrile-0.1%phosphoric acid aqueous solution as mobile phase,gradient elution;flow rate was 0.2 mL/min;the detection wavelength was 266 nm for the first 30 minutes and 235 nm for the last 36 minutes;the column temperature was 30℃.The UPLC fingerprint of Linggui Zhugan Decoction was established by Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012.130723 version),and the common peak was determined and the similarity evaluation was carried out.Based on the peak area determination results of the common peak of the fingerprint,the quality of different batches of Linggui Zhugan Decoction was evaluated by chemometrics such as clustering analysis and principal component analysis.Results A total of 24 common peaks were confirmed and 14 components were identified by using reference substances.The similarity of 10 batches of Linggui Zhugan Decoction samples was greater than 0.950,which could be divided into two categories by chemometrics,and the principal component 1-4 were the main factors affecting its quality evaluation.OPLS-DA identified 6 differential markers.Conclusion The fingerprint research method established in the study is simple,reliable and reproducible.Through the method of fingerprint combined with chemometrics analysis,the differences between Linggui Zhugan Decoction from different origins of medicinal materials are identified,which provides a reference for the internal quality evaluation of Linggui Zhugan Decoction.关键词
苓桂术甘汤/超高效液相色谱法/指纹图谱/化学计量学/聚类分析/主成分分析/正交偏最小二乘-判别分析Key words
Linggui Zhugan Decoction/UPLC/fingerprint/chemometrics/clustering analysis/principal component analysis/orthogonal partial least square discriminant analysis分类
医药卫生引用本文复制引用
刘芳洁,栾真杰,何纯莲,王希霖,左培..基于UPLC指纹图谱结合化学计量学的经典名方苓桂术甘汤质量评价研究[J].中国中医药信息杂志,2024,31(1):128-134,7.基金项目
湖南省自然科学基金面上项目(2022JJ30410) （2022JJ30410）
