中国中西医结合外科杂志2023，Vol.29Issue(6)：814-818,5.DOI:10.3969/j.issn.1007-6948.2023.06.017
电针通过NLRP3/caspase-1通路介导的细胞焦亡途径减轻脓毒症大鼠急性肺损伤
Electroacupuncture attenuates acute lung injury induced by sepsis via NLRP3/caspase-1 pathway mediated pyrocytosis pathway
摘要
Abstract
Objective To observe whether electroacupuncture at Zusanli(ST36)and Feishu(BL13)acupoints reduces sepsis induced acute lung injury in rats through the pyroptosis pathway mediated by NLRP3/caspase-1 pathway.Methods Thirty adult healthy male SD rats were randomly divided into five groups:control group,model group,electroacupuncture group,electroacupuncture plus NLRP3 activator group,and non-acupoint electroacupuncture group,with six rats in each group.The sepsis model was established by cecal ligation and puncture.In the electroacupuncture group,bilateral ST36 and BL13 acupoints were stimulated with dense and sparse wave electroacupuncture(once daily for 5 days before model preparation,once every 6 hours during model preparation,30 minutes each time).In the non-acupoint electroacupuncture group,the same parameters of electroacupuncture were applied at non-acupoints located 5 mm away from ST36 and BL13.The electroacupuncture plus NLRP3 activator group received intraperitoneal injection of 100 mg/kg nigericin sodium salt 30 minutes before model preparation.After modeling,the rats were sacrificed at 24 hours,and lung tissues were collected to calculate the wet-to-dry weight ratio(W/D ratio),observe pathological changes,and evaluate lung injury scores.The levels of IL-1β,IL-18 in lung tissues were measured by ELISA,and the expression levels of NLRP3,ASC,cleaved caspase-1,and GSDMD-N proteins in lung tissues were detected by Western blot.Results Compared with the control group,the lung injury scores,W/D ratio,IL-1 β and IL-18 levels in the model group,electroacupuncture group,electroacupuncture plus NLRP3 activator group,and non-acupoint electroacupuncture group were increased.The expressions of NLRP3,ASC,cleaved caspase-1,and GSDMD-N proteins in lung tissues were upregulated,and the differences were statistically significant(P＜0.05).Compared with the model group,the lung injury scores,W/D ratio,IL-1β and IL-18 levels in the electroacupuncture group were decreased.The expressions of NLRP3,ASC,cleaved caspase-1,and GSDMD-N proteins in lung tissues were downregulated,and the differences were statistically significant(P＜0.05).Compared with the electroacupuncture group,the lung injury scores,W/D ratio,IL-1β and IL-18 levels in the electroacupuncture plus NLRP3 activator group were increased.The expressions of NLRP3,ASC,cleaved caspase-1,and GSDMD-N proteins in lung tissues were upregulated,and the differences were statistically significant(P＜0.05).Conclusion Electroacupuncture stimulation at ST36 and BL13 acupoints may alleviate acute lung injury in rats with sepsis through inhibiting the classical cell pyroptosis pathway mediated by NLRP3/caspase-1 pathway.关键词
电刺激疗法/肺损伤/细胞焦亡Key words
Electrical acupoint stimulation/lung injury/pyroptosis分类
医药卫生引用本文复制引用
张昊,苏乾,史佳,宫丽荣..电针通过NLRP3/caspase-1通路介导的细胞焦亡途径减轻脓毒症大鼠急性肺损伤[J].中国中西医结合外科杂志,2023,29(6):814-818,5.基金项目
国家自然科学基金青年项目(82004076) （82004076）
