中医药学报2024，Vol.52Issue(1)：70-76,7.DOI:10.19664/j.cnki.1002-2392.240013
基于TMT标记定量蛋白质组学技术研究老龄肾阳虚证患者血浆差异蛋白的变化及意义
Changes and Significance of Plasma Differential Proteins in Aged Patients with Kidney-yang Deficiency Syndrome Were Studied Based on TMT-Labeled Quantitative Protein Omics
摘要
Abstract
Objective:To excavate the changes and significance of plasma differentially expressed proteins in aged patients with kidney-yang deficiency syndrome based on TMT-labeled quantitative protein omics.Methods:The plasma samples of eight aged patients with kidney-yang deficiency syndrome in line with TCM syndrome differentiation and eight healthy people of the same age were recruited,and three plasma samples were randomly selected from each group.Protein quantitative analysis was performed using TMT protein omics technology and the differentially expressed proteins were screened out,followed by GO enrichment and KEGG enrichment analysis for the differentially expressed proteins.The important proteins of the differentially expressed proteins were screened out by protein interaction network analysis,and the proteins that interacted with Alzheimer's disease(AD)were tested with ELISA in another five aged patients with kidney-yang deficiency and five healthy people of the same age.Results:A total of 83 differentially expressed proteins were obtained in the aged kidney-yang deficiency patient group and the control group of healthy people of the same age.73 of the 83 differentially expressed proteins were up-regulated and 10 were down-regulated.Differentially expressed proteins involve biological processes,such as glycolysis and protein folding,cellular components,such as cytoplasm and extracellular space,and molecular functions,such as cadherin binding and protein binding.These proteins also involves pyruvate metabolism,AD,estrogen signaling pathway,glucagon signaling pathway,etc.Protein validation related to the occurrence and development of AD showed that calpain 1 and α-synuclein expressions in the plasma of the aged patients with kidney-yang deficiency were significantly higher than those in the control group(P<0.05).Conclusion:There were differentially expressed proteins in plasma between the aged patients with kidney-yang deficiency and healthy people of the same age group,and among these differentially expressed proteins,calpain 1 and α-synuclein were related to the occurrence and development of AD.This finding provides a molecular mechanism for exploring the vulnerability of aged patients with kidney-yang deficiency to AD,and plays an important role in the prevention of disease by traditional Chinese medicine.关键词
肾阳虚/蛋白质组学/衰老/阿尔茨海默病Key words
Kidney yang deficiency/Proteomics/Aging/Alzheimer's disease分类
医药卫生引用本文复制引用
管亚男,丛培玮,赵丹玉,任路,王旭..基于TMT标记定量蛋白质组学技术研究老龄肾阳虚证患者血浆差异蛋白的变化及意义[J].中医药学报,2024,52(1):70-76,7.基金项目
国家重点研发计划中医药现代化研究重点专项分课题(2018YFC1704300) （2018YFC1704300）
