中国中医药信息杂志2024，Vol.31Issue(1)：152-158,7.DOI:10.19879/j.cnki.1005-5304.202307070
基于16S rDNA测序技术的膝骨关节炎湿热痹阻证、寒湿痹阻证患者肠道菌群差异性研究
Differential Study on Intestinal Flora between Knee Osteoarthritis with Dampness-heat Obstruction Syndrome and Cold-dampness Blockage Syndrome Based on 16S rDNA Sequencing Technology
摘要
Abstract
Objective To compare the differences in the composition and diversity of intestinal flora between knee osteoarthritis(KOA)patients with dampness-heat obstruction syndrome,cold-dampness blockage syndrome and healthy people;To explore the characteristics of intestinal flora of KOA patients with the above two TCM syndrome types.Methods According to the inclusion and exclusion criteria,dampness-heat obstruction syndrome group,cold-dampness blockage syndrome group,and healthy control group were screened,with 10 cases for each group,and then their fecal samples were collected.16S rDNA sequencing technology was used to compare the differences of intestinal flora among groups by Alpha and Beta diversity analysis.Results There was no significant difference in species richness of intestinal flora among the three groups,but there was statistical significance in species diversity between cold-dampness blockage syndrome group and dampness-heat obstruction syndrome group,and healthy control group(P<0.05).There were statistical significance among the three groups in intestinal flora composition(P= 0.001).At the phylum level,Bacteroidota and Firmicutes were significantly dominant,and at genus level,the abundance of Prevotella in dampness-heat obstruction syndrome group and cold-dampness blockage syndrome group increased.The abundance of Enterobacteriaceae,Lachnospira and Klebsiella was relatively large in dampness-heat obstruction syndrome group.The abundance of Prevotella and Pseudomonas in cold-dampness blockage syndrome group was large.Conclusion There are differences in the intestinal flora structure between KOA patients with dampness-heat obstruction syndrome and cold-dampness blockage syndrome.关键词
膝骨关节炎/湿热痹阻证/寒湿痹阻证/肠道菌群/16S rDNAKey words
knee osteoarthritis/dampness-heat obstruction syndrome/cold-dampness blockage syndrome/intestinal flora/16S rDNA分类
医药卫生引用本文复制引用
叶敏兰,唐晓颇,高阳鹭,王建..基于16S rDNA测序技术的膝骨关节炎湿热痹阻证、寒湿痹阻证患者肠道菌群差异性研究[J].中国中医药信息杂志,2024,31(1):152-158,7.基金项目
中国中医科学院科技创新工程项目(CI2021A01511) （CI2021A01511）
