中国肿瘤生物治疗杂志2023，Vol.30Issue(12)：1061-1065,5.DOI:10.3872/j.issn.1007-385x.2023.12.004
甘草查尔酮B对三阴性乳腺癌MDA-MB-231细胞的抑制作用及其机制
The inhibitory effect of Licochalcone B on the triple negative breast cancer cell MDA-MB-231 and its mechanism
摘要
Abstract
Objective:To investigate the inhibitory effect of Licochalcone B(LCB)on a triple-negative breast cancer cell line MDA-MB-231 cells and its mechanism.Methods:MDA-MB-231 cells were routinely cultured and treated with different concentrations of LCB,and then CCK-8 assay,immunofluorescence,FCM and WB analysis were used to detect the proliferative viability of MDA-MB-231 cells,the expression of γ-H2AX,a marker of DNA double-strand breaks in the nucleus,the cell cycle,and the expression levels of proteins related to cycle regulation,mitogen-activated protein kinase(MAPK),and the endoplasmic reticulum stress(ER stress),respectively.Results:LCB significantly inhibited the proliferative viability of breast cancer MDA-MB-231 cells(all P<0.05),the number of γ-H2AX-positive cells and protein expression levels were significantly increased after LCB treatment(all P<0.05),the number of cells in G2/M and S phases was significantly increased(all P<0.05),the phosphorylation levels of the main members of the MAPK family,extracellular regulated kinase 1/2(ERK1/2)and p38 MAPK phosphorylation levels were significantly upregulated(all P<0.05),and the expression of ER stress pathway-related proteins Bip,ATF4 and CHOP were significantly upregulated(all P<0.05).Conclusion:LCB could significantly inhibit the proliferation vitality of MDA-MB-231 cells and induce DNA damage and cell cycle arrest at G2/M and S phases.The inhibitory effect of LCB on MDA-MB-231 cells may be related to the activation of MAPK and ER stress signaling pathway.关键词
甘草查尔酮B/三阴性乳腺癌/MDA-MB-231细胞/增殖/DNA损伤/细胞周期阻滞/丝裂原活化蛋白激酶/内质网应激Key words
Licochalcone B(LCB)/triple-negative breast cancer(TNBC)/MDA-MB-231 cell/proliferation/DNA damage/cell cycle arrest/mitogen-activated protein kinase(MAPK)/endoplasmic reticulum stress(ER stress)分类
医药卫生引用本文复制引用
王琳琳,曹露,杨洪川,王凤泽,吴开祥..甘草查尔酮B对三阴性乳腺癌MDA-MB-231细胞的抑制作用及其机制[J].中国肿瘤生物治疗杂志,2023,30(12):1061-1065,5.基金项目
山东省自然科学基金(No.ZR2018MH026) （No.ZR2018MH026）
泰安市科技创新发展项目(No.2022NS322,No.2022NS353) （No.2022NS322,No.2022NS353）
