中国肿瘤生物治疗杂志2023，Vol.30Issue(12)：1066-1073,8.DOI:10.3872/j.issn.1007-385x.2023.12.005
miR-4465通过靶向下调HMGA1的表达抑制肝细胞癌Hep3B细胞的恶性生物学行为
miR-4465 inhibits the malignant biological behaviors of hepatocellular carcinoma Hep3B cells by targeting and downregulating the expression of HMGA1
摘要
Abstract
Objective:To investigate the effect of miR-4465 targeting high mobility group protein A1(HMGA1)on the proliferation,migration and invasion of hepatocellular carcinoma Hep3B cells.Methods:Sixteen pairs of cancer tissues and adjacent tissue samples from patients diagnosed with hepatocellular carcinoma in the First Affiliated Hospital of Wannan Medical College from May 2020 to September 2021 were collected.The expression of miR-4465 in hepatocellular carcinoma tissues and Hep3B and Huh7 cells was analyzed by qPCR,and the regulatory relationship between miR-4465 and HMGA1 was verified by dual luciferase reporter gene assay.According to the different transfections,Hep3B cells were group,si-NC group,and si-HMGA1 group for transfection;in addition,mimics-NC+pcDNA-NC,miR-4465 mimics+pcDNA-NC,and miR-4465 mimics+pcDNA-HMGA1 were transfected for rescue experiments.The changes of mRNA and protein levels of HMGA1 in each group were detected by qPCR and WB assay;the changes in cell proliferation activity in each group were detected by CCK-8 method;the changes in cell migration ability in each group were detected by scratch assay,and the changes in cell invasion ability in each group were detected by Transwell assay.Results:The expression of miR-4465 in hepatocellular carcinoma tissues and cells was significantly lower than that in adjacent tissues and normal liver cells(P<0.05 or P<0.001).After transfection for 48 h,the proliferation,migration and invasion abilities of Hep3B cells overexpressing miR-4465 were significantly decreased(P<0.05,P<0.01 or P<0.001);the proliferation,migration and invasion abilities of Hep3B cells with miR-4465 knockdown were increased(P<0.05,P<0.01 or P<0.001).The binding relationship between HMGA1-3'UTR and miR-4465 was verified by dual luciferase report gene experiment.miR-4465 could target and down-regulate the mRNA and protein expression of HMGA1(all P<0.01).Over-expression of HMGA1 partially restored the inhibitory effects of miR-4465 overexpression on cell proliferation,migration and invasion as well as HMGA1 expression(all P<0.05).Conclusion:miR-4465 inhibits the malignant biological behaviors of hepatocellular carcinoma Hep3B cells by down-regulating the expression of HMGA1 in the cells.关键词
miR-4465/高迁移率族蛋白A1/肝细胞癌/Hep3B细胞/增殖/迁移/侵袭Key words
miR-4465/high mobility group protein A1(HMGA1)/hepatocellular carcinoma/Hep3B cell/proliferation/migration/invasion分类
医药卫生引用本文复制引用
张博超,马思源,朱萍,赵晓晓,王成,刘静文,蒋平,浦春..miR-4465通过靶向下调HMGA1的表达抑制肝细胞癌Hep3B细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志,2023,30(12):1066-1073,8.基金项目
国家自然科学基金项目(No.81772180) （No.81772180）
安徽省教育厅省级自然科学重点项目(No.KJ2016A722) （No.KJ2016A722）
安徽省大学生创新训练项目(No.s202110368042) （No.s202110368042）
