中国肿瘤生物治疗杂志2023，Vol.30Issue(12)：1074-1081,8.DOI:10.3872/j.issn.1007-385x.2023.12.006
小核核糖核蛋白多肽A对肝细胞癌细胞恶性生物学行为的影响及其机制
Effect of small nuclear ribonucleoprotein polypeptide A on the malignant biological behavior of hepatocellular carcinoma cells and its mechanism
摘要
Abstract
Objective:To investigate the expression of SNRPA in hepatocellular carcinoma(HCC)and cells and the role and mechanism of small nuclear ribonucleoprotein polypeptide A(SNRPA)in regulating the malignant biological behaviors of HCC HepG2 and Hep3B cells.Methods:The database was used to analyze the expression of SNRPA in pan-cancer tissues and its correlation with the pathological stage and the prognosis of HCC patients.HepG2 and Hep3B cells were routinely cultured.si-NC,si-SNRPA#1,si-SNRPA#2 were transfected into HepG2 and Hep3B cells and recorded as si-NC,si-SNRPA#1 and si-SNRPA#2 group.SNRPA-vectors and SNRPA-oe vectors were transfected into LO2 cells and recorded as SNRP-vector and SNRPA-oe group.qPCR was used to detect the expression of SNRPA mRNA in normal hepatocytes and HCC cells,as well as HepG2 and Hep3B cells transfected with each group.MTT,Transwell and WB assays were used to respectively investigate the changes in the proliferation,migration and invasion abilities as well as the expression of EMT-related proteins in the transfected HepG2 and Hep3B cells in each group.Results:Database analysis showed that SNRPA mRNA was highly expressed in the majority of tumors(all P<0.001)and correlated with their pathological stages(P<0.05 or P<0.01).SNRPA was highly expressed in both HCC tissues and HCC cells(P<0.05 or P<0.01)and was correlated with the prognosis of HCC patients(P<0.01).Knockdown of SNRPA expression significantly inhibited the proliferation of HepG2 and Hep3B cells(P<0.05 or P<0.01)while overexpression of SNRPA promoted the proliferation of LO2 cells(P<0.01).Knockdown of SNRPA expression significantly inhibited the migration and invasion abilities of HepG2 and Hep3B cells(both P<0.01)and promoted a marked up-regulation of the expression of E-cadherin(P<0.01)and suppressed the expression of N-cadherin and vimentin(P<0.01).Conclusion:The expression of SNRPA was significantly elevated in HCC tissues and cells,and it may promote the proliferation,migration and invasion of HepG2 and Hep3B cells by regulating the epithelial-mesenchymal transition(EMT)process.关键词
肝细胞癌/HepG2细胞/Hep3B细胞/小核核糖核蛋白多肽A/增殖/迁移/侵袭/上皮间质转化Key words
hepatocellular carcinoma(HCC)/HepG2 cell/Hep3B cell/small nuclear ribonucleoprotein polypeptide A(SNRPA)/proliferation/migration/invasion/epithelial-mesenchymal transition(EMT)分类
医药卫生引用本文复制引用
姚梦琳,王如画,崔小萌,陈昳菲,郭丹,和水祥,李雅睿..小核核糖核蛋白多肽A对肝细胞癌细胞恶性生物学行为的影响及其机制[J].中国肿瘤生物治疗杂志,2023,30(12):1074-1081,8.基金项目
西安交通大学第一附属医院科研发展基金(No.2020QN-12) （No.2020QN-12）
