中南民族大学学报（自然科学版）2024，Vol.43Issue(1)：24-31,8.DOI:10.20056/j.cnki.ZNMDZK.20240104
一株内切葡聚糖酶产生菌株的分离鉴定及其产酶条件优化
Isolation and identification of an endoglucanase producing strain and optimization of its enzymatic production conditions
摘要
Abstract
A strain with high production of endoglucanase was selected from the straw fermentation of cattle stomach in Changsha County,Hunan Province,and was named SCUEC7.Based on the morphological observation,physiological and biochemical characteristics,and 16S rDNA sequence comparison analysis,SCUEC7 strain was identified as Bacillus subtilis.The culture conditions for the better growth of SCUEC7 strain were as follows:12 h of culture period,pH 6.0,37℃of culture temperature,20.0 g·L-1 maltose as carbon source,15.0 g·L-1 tryptone as nitrogen source,1.5 g·L-1 Mn2+ as metal ion.Response surface methodology analysis showed that the highest predicted enzyme activity was 409.0 U·mL-1 when the initial pH value was 5.9,maltose content was 19.8 g·L-1,and culture period was 11.8 h.There was a high degree of fit between the measured and predicted values of endoglucanase activity.SCUEC7 strain has a strong ability to produce endoglucanase,which can provide a basis for the development of animal feed.关键词
枯草芽胞杆菌SCUEC7菌株/内切葡聚糖酶/产酶条件优化/响应面法优化Key words
Bacillus subtilis SCUEC7 strain/endoglucanase/optimization of enzymatic production conditions/response surface optimization分类
生物科学引用本文复制引用
赵思卡,许倍滔,冯喆,王力,张建国,李晓华..一株内切葡聚糖酶产生菌株的分离鉴定及其产酶条件优化[J].中南民族大学学报（自然科学版）,2024,43(1):24-31,8.基金项目
国家自然科学基金资助项目(31070087) （31070087）
中央高校基本科研业务费专项资金资助项目(CZY19018) （CZY19018）
中南民族大学企业委托资助项目(HZY20042,HZY21015) （HZY20042,HZY21015）
