楸树DELLA基因家族生信分析及CbuGRAS9的功能分析OACSTPCD

To provide theoretical basis for genetic improvement of reproductive regulatory traits of Catalpa bungei,DELLAs family genes were identified and the function of CbuGRAS9 was analyzed.Based on the genomic data of Catalpa bungei,five CbuDELLAs genes homologous to Arabidopsis thaliana were identified and cloned.ExPASy,SWISS-MODEL,Plant-mPloc,PlantCare and other online tools were used to predict the isoelectric point,protein structure,sub-cellular localization and promoter cis-acting elements of CbuDELLAs protein.The expression differences of CbuDELLAs were analyzed by using Catalpa'Bairihua'and Catalpa bungei'Luoqiu No.1'as materials,and the molecular function of CbuGRAS9 was confirmed by heterologus transformation in Arabidopsis thaliana,and the proteins interacted with CbuGRAS9 were screened by yeast two-hybrid library.The results showed that the amino acid number of the five CbuDELLAs proteins ranged from 455 to 588 aa,the relative molecular weight of the proteins ranged from 5.04 to 6.43 kDa,and the isoelectric point value ranged from 4.81 to 5.14.All CbuDELLAs proteins contained DELLA and GRAS conserved domains and are hydrophilic proteins.Sub-cellular localization prediction showed that CbuDELLAs protein was located in the nucleus.The analysis of promoter cis-acting elements showed that the five promoter regions of DELLAs all contained cis-acting elements involved in gibberellin reaction.The results of qRT-PCR showed that the expression of CbuDELLAs in Catalpa'Bairihua'were significantly higher than that in Catalpa bungei'Luoqiu No.1',and CbuGRAS9 was the most significantly gene,and the flowering time of CbuGRAS9 transgenic plants were significantly delayed.Proteins interacted with CbuGRAS9 were mainly concentrated in metabolic pathways such as ribosome,amino acid synthesis,secondary metabolism,photosynthesis and TCA cycle.