中国中西医结合杂志2023，Vol.43Issue(12)：1433-1441,9.DOI:10.7661/j.cjim.20230517.079
柴胡加龙骨牡蛎汤对2型糖尿病合并抑郁患者肠道菌群的影响
Effect of Chaihu plus Longgu Muli Decoction on Intestinal Microflora of Type 2 Diabetes Patients with Depression
摘要
Abstract
Objective To observe the effects of Chaihu plus Longgu Muli Decoction(CLMD)on intestinal microflora structural characteristics of patients with type 2 diabetes mellitus complicated with depression.Methods A total of 86 patients with type 2 diabetes mellitus complicated with depression were randomly divided into the experimental group and control group,with 43 patients in each group.The control group was orally treated with flupentixol melitracen tablets(Deanxit),twice a day,one tablet in the morning and one tablet at noon,the experimental group received Deanxit combined with CLMD twice a day,one bag each time.The intervention course remained 8 weeks.The changes of fasting blood glucose(FPG),postprandial blood glucose(2hPG),glycated albumin(GA),interleukin-6(IL-6),C-reactive protein(CRP),Hamilton Depression Scale(HAMD-17)score,Self-Rating Depression Scale(SDS)score and Chinese medicine(CM)syndrome score were compared between the two groups before and after treatment.The OUT number,diversity index and differential flora of intestinal flora were comprehensively analyzed and evaluated by 16S rDNA high-throughput sequencing.Results Compared with before treatment,the levels of FPG,2hPG,GA,IL-6 and CRP in two groups significantly decreased after treatment(P＜0.05),5-HT level increased(P＜0.05),the improvement degree of GA,IL-6,CRP and 5-HT in the experimental group was better than those in control group(P＜0.05).Compared with the control group after treatment,HAMD-17 score and CM syndrome score of experimental group significantly decreased(P＜0.05).Alpha diversity analysis showed that Shannon index,Chao index and Ace index increased significantly after treatment(P＜0.05),Simpson index significantly decreased(P＜0.05).Beta diversity analysis showed that after treatment,the distribution location and quadrant range of intestinal microflora in the experimental group changed significantly compared with the baseline.LefSe difference analysis showed that there were 4 differentially divided phyla,7 differentially divided families and 9 differentially divided genera in the experimental group before and after treatment.Compared with the control group at the same time,the abundance of Firmicutes was mainly increased.The abundance of Proteobacteria and Bacteroidetes decreased.Conclusion CLMD can effectively improve the clinical symptoms of type 2 diabetes patients with depression,and the mechanism may be related to down-regulation of inflammatory cytokines,up-regulation of 5-HT concentration and regulation of intestinal microflora structure.关键词
柴胡加龙骨牡蛎汤/肠道菌群/抑郁/2型糖尿病/中药复方/中西医结合Key words
Chaihu plus Longgu Muli Decoction/intestinal microflora/depression/type 2 diabetes/Chinese herbal compound/integrative meidicine引用本文复制引用
李宇,蔡萧君,王雪,李浩经,张卓辉,吴圆圆,王钦..柴胡加龙骨牡蛎汤对2型糖尿病合并抑郁患者肠道菌群的影响[J].中国中西医结合杂志,2023,43(12):1433-1441,9.基金项目
黑龙江省应用技术研究与开发计划项目(No.GA19C108) （No.GA19C108）
