摘要
Abstract
Objective To investigate the preventive effect of the compound of Ginseng flower bud and Astragalus on primary osteoporosis in aging male rats.Methods Sixty male SD rats,with 3 months old were randomly divided into 6 groups:the control group(NC group),D-galactose model group(DG group),positive control group(SP group,Stanazol+Piracetam),Chinese medicine control group(GB group,Xianling Gubao Capsule),the low-dose group of the compound of Ginseng Flower Bud and Astragalus(GFRAL group),and the high dose group of the compound of Ginseng Flower Bud and Astragalus(GFRAH group),10 in each group.After 3 months of continuous administration,all the rats were sacrificed.Then the left tibias were taken for bone histophiometry,the right femurs were used to detect biomechanical properties,and the left femurs were taken to measure bone calcium,phosphate and hydroxyproline(Hyp)concentrations.Results Compared with NC group,the static parameters of percent trabecular area(％Tb.Ar)and trabecular number(Tb.N)in upper tibia of DG group decreased,and the degree of trabecular spacing(Tb.Sp)increased(P＜0.05,P＜0.01).While osteoclasts number(Oc.N)and ostelclast perimeter/Tb.Pm x 100(％Oc.Pm)per trabecular area of the dynamic parameters of upper tibia segment increased(P＜0.01),mineral appositeion rate(MAR),bone formation rate of bone surface(BFR/BS),bone formation rate(BFR/BV),bone formation rate of trabecular volume(BFR/TV)decreased(P＜0.05),and femoral Hyp decreased(P＜0.01).Compared with DG group,the static parameters of％Tb.Ar,Tb.Th and Tb.N in upper tibia in GFRAH and GFRAL groups increased(P＜0.01,P＜0.05),and Tb.Sp decreased(P＜0.05),respectively.The Hyp of femur in GFRAL group decreased(P＜0.05).Conclusions The compound of Ginseng flower bud and Astragalus has similar preventive effect with compound Stanazol and Xianling Gubao Capsule.It can effectively prevent osteoporosis in male rats caused by D-galactose,and it is suitable for the elderly to use in daily life.could effectively prevent from osteoporosis caused by D-galactose in male aging rats,and the compound may have the potential to become an ideal anti-osteoporosis drug.关键词
人参花蕾黄芪合剂/D-半乳糖/雄性大鼠/骨质疏松/中成药Key words
compound of Ginseng flower bud and Astragalus/D-galactose/male rats/osteoporosis/Chinese herbal compound
