福建农业科技2023，Vol.54Issue(10)：15-22,8.DOI:10.13651/j.cnki.fjnykj.2023.10.003
基于冷冻电镜的草鱼呼肠孤病毒对称失配三维重构算法研究
Study on the Three-dimensional Reconstruction Algorithm of Symmetric Mismatch of Grass Corp Reovirus Based on Cryo-EM
童江杨 1陈建楠 2薛婷 2王勖荣1
作者信息
- 1. 福建师范大学生命科学学院,福建 福州 350117
- 2. 福建师范大学生命科学学院,福建 福州 350117||福建省特色海洋生物资源可持续利用重点实验室,福建 福州 350117||海洋生物医药与制品产业化开发技术公共服务平台,福建 福州 350117
- 折叠
摘要
Abstract
In the process of reconstructing the three-dimensional structure of the virus by using the icosahedral symme-try of the virus,the existence of the symmetry-mismatch problem hindered the analysis of the real structure of the vi-rus and the acquisition of the high-resolution structure.In order to eliminate the influence of symmetric mismatch on the three-dimensional structure of the virus,a symmetry-mismatch three-dimensional reconstruction algorithm by u-sing the special subgroup search of the icosahedral point group was proposed.By taking the Grass Corp Reovirus(GCRV)as the experimental material,the three-dimensional structure of GCRV was obtained by using the three-di-mension reconstruction technique of cryo-electron microscopy.Then,the orientation information was obtained through the local three-dimensional reconstruction and symmetry-mismatch searching,by adding the search of GCRV-specific vertex.The results showed that:the algorithm improved the efficiency of symmetry-mismatch orientation searching,and accurately reduced the orientation information of Grass Corp Reovirus(GCRV)by determining the icosahedral-specific apical position of the polymerase complex inside the virus.The results confirmed that the virus-deleted RdRp was in the pseudo-D3 symmetrical equatorial group,and it provided a new method for resolving the whole-virus symmetry-mismatch structure of double-stranded RNA virus and exploring the molecular mechanism of endogenous transcription of double-stranded RNA virus.关键词
草鱼呼肠孤病毒/三维结构/对称失配/聚合酶Key words
Grass corp reovirus/Three-dimensional structure/Symmetric mismatch/Polymerase分类
农业科技引用本文复制引用
童江杨,陈建楠,薛婷,王勖荣..基于冷冻电镜的草鱼呼肠孤病毒对称失配三维重构算法研究[J].福建农业科技,2023,54(10):15-22,8.
