福建农业科技2023，Vol.54Issue(10)：29-33,5.DOI:10.13651/j.cnki.fjnykj.2023.10.005
UFLC法测定不同地区博落回果实中生物碱的含量
Determination of Alkaloid Contents in the Fruits of Macleaya cordata in Different Regions by UFLC Method
摘要
Abstract
In order to screen the germplasm resources of Macleaya cordata with high quality and promote the breeding of excellent varieties of M.cordata,the contents of the four kinds of alkaloid such as fumarine,β-homocheliconine,sanguinarine and chelerythrine in the fruits of 16 M.cordata resources from 15 different regions were determined by UFLC method,thus to explore the difference of alkaloid contents in the fruits of M.cordata from different regions.The results showed that there were differences among the fumarine,β-homocheliconine,sanguinarine and cheleryth-rine in the fruits of M.cordata from 15 regions,as well as the total contents of the four kinds of alkaloid.Among them,the total alkaloid content of MC-1 M.cordata in Mingxi County,Sanming City,Fujian Province was the high-est,reaching(0.435 2±0.008 8)％,which was significantly different from those of the samples in other regions.The contents of β-homocheliconine,sanguinarine and chelerythrine in MC-1.cordata were the highest,and the trend of the alkaloid contents was β-homocheliconine＞sanguinarine＞fumarine＞chelerythrine,indicating that MC-1 could be used as the germplasm resource for breeding the local excellent varieties of M.cordata.The results of this study would provide theoretical basis for the breeding work of M.cordata.关键词
博落回/超快速液相色谱(UFLC)法/生物碱/果实/不同地区Key words
Macleaya cordata/Ultra-fast liquid chromatography(UFLC)method/Alkaloid/Fruit/Different regions分类
农业科技引用本文复制引用
郑梅霞,苏海兰,李炽勤,朱育菁,陈宏,牛雨晴,朱雁鸣..UFLC法测定不同地区博落回果实中生物碱的含量[J].福建农业科技,2023,54(10):29-33,5.基金项目
国家自然科学基金项目(31972335) （31972335）
福建省科技计划重大专项(2022NZ029017) （2022NZ029017）
福建省科技计划公益类项目(2020R1034008、2022R1034008) （2020R1034008、2022R1034008）
国家重点研发计划(2022YFD1600300) （2022YFD1600300）
福建省财政专项——福建省农业科学院科技创新团队建设项目(CXTD2021014-2、2022KF21). （CXTD2021014-2、2022KF21）
