福建农业科技2023，Vol.54Issue(10)：34-39,6.DOI:10.13651/j.cnki.fjnykj.2023.10.006
利用基因编辑技术创制福香占糯稻新种质
Creation of Novel Glutinous Rice Germplasm of Fuxiangzhan(FXZ)Using Genome Editing
摘要
Abstract
Gene editing is an emerging genetic improvement technology that allows for rapid and precise modification of crop traits.FXZ is a newly developed high-quality,blast-resistant,long-grain indica rice variety with great potential for commercialization.This study aimed to utilize gene editing technology to create FXZ glutinous rice materials rapid-ly.By screening and optimizing target sequences,the rice granule-bound starch synthase gene(Waxy,Wx)was suc-cessfully knocked out using CRISPR/Cas9 technology,resulting in Wx gene knockout mutants.Through progeny ag-ronomic traits evaluation and amylose content determination,the results showed that the amylose content of the knockout materials decreased from 16.3％to approximately 1.67％-1.81％,meeting the criteriaof glutinous rice.Additionally,there were no significant changes observed in plant morphology,yield,and resistance in the mutant plants.This study provides a new approach for the application and promotion of FXZ and enriches the genetic re-sources of blast-resistant glutinous rice.关键词
基因编辑/糯稻/福香占/颗粒结合型淀粉合酶基因Key words
Gene editing/Glutinous rice/Fuxiangzhan(FXZ)/Granule-bound starch gynthase gene分类
农业科技引用本文复制引用
魏林艳,樊佳星,张敏,王进兰,谢鸿光,魏毅东,张建福..利用基因编辑技术创制福香占糯稻新种质[J].福建农业科技,2023,54(10):34-39,6.基金项目
福建省科技计划公益类项目(2023R1021001) （2023R1021001）
福建省科技重大专项(2022NZ030014) （2022NZ030014）
福建省科技计划项目——中央引导项目(2022L3018). （2022L3018）
