法医学杂志2023，Vol.39Issue(6)：535-541,7.DOI:10.12116/j.issn.1004-5619.2023.430317
傅里叶变换红外光谱术用于急性及陈旧性心肌梗死的法医病理学诊断
Forensic Pathological Diagnosis of Acute and Old Myocardial Infarction Using Fourier Transform Infrared Spectroscopy
摘要
Abstract
Objective Fourier transform infrared spectroscopy(FTIR)was used to analyze myocardial infarction tissues at different stages of pathological change to achieve the forensic pathology diagnosis of acute and old myocardial infarction.Methods FTIR spectra data of early ischemic myocardium,ne-crotic myocardium,and myocardial fibrous tissue in the left ventricular anterior wall of the sudden death group of atherosclerotic heart disease and the myocardium of the normal control group were col-lected using hematoxylin-eosin(HE)and immunohistochemistry(IHC)staining as a reference,and the data were analyzed using multivariate statistical analysis.Results The mean normalized spectra of control myocardium,early ischemic myocardium and necrotic myocardium were relatively similar,but the mean second derivative spectra were significantly different.The peak intensity of secondary struc-ture of proteins in early ischemic myocardium was significantly higher than in other types of myocar-dium,and the peak intensity of the α-helix in necrotic myocardium was the lowest.The peaks of amide Ⅰ and amide Ⅱ in the mean normalized spectra of myocardial fibrous tissue significantly shifted towards higher wave numbers,the peak intensities of amide Ⅱ and amide Ⅲ were higher than those of other types of myocardium,and the peak intensities at 1 338,1 284,1 238 and 1 204 cm-1 in the mean second derivative spectra were significantly enhanced.Principal component analysis(PCA)and partial least square-discriminant analysis(PLS-DA)showed that FTIR could distinguish different types of myocardium.Conclusion FTIR technique has the potential to diagnose acute and old myocardial in-farction,and provides a new basis for the analysis of the causes of sudden cardiac death.关键词
法医病理学/傅里叶变换红外光谱术/急性心肌梗死/陈旧性心肌梗死/主成分分析/偏最小二乘-判别分析Key words
forensic pathology/Fourier transform infrared spectroscopy(FTIR)/acute myocardial infarc-tion/old myocardial infarction/principal component analysis/partial least square-discriminant analysis分类
特种医学引用本文复制引用
田甜,廖信彪,张付,邓恺飞,张吉,黄平,陈忆九,张建华..傅里叶变换红外光谱术用于急性及陈旧性心肌梗死的法医病理学诊断[J].法医学杂志,2023,39(6):535-541,7.基金项目
国家重点研发计划资助项目(2022YFC3302002) （2022YFC3302002）
国家自然科学基金资助项目(82072115) （82072115）
中央级公益性科研院所资助项目(GY2022G-3) （GY2022G-3）
上海市法医学重点实验室资助项目(21DZ2270800) （21DZ2270800）
上海市司法鉴定专业技术服务平台资助项目 （）
法医病理学公安部重点实验室开放课题资助项目(GAFYBL202206) （GAFYBL202206）
