法医学杂志2023，Vol.39Issue(6)：579-585,7.DOI:10.12116/j.issn.1004-5619.2022.520902
IDentifier DNA分型盒(炎黄34)的法医学验证及应用评估
Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit(Yan-Huang34)
摘要
Abstract
Objective To investigate the technical performance of IDentifier DNA typing kit(YanHuang34)and evaluate its forensic application value.Methods Following the Criterion of Forensic Science Hu-man Fluorescence STR Multiplex Amplification Reagent(GB/T 37226-2018),IDentifier DNA typing kit(YanHuang34)was verified in 11 aspects of species specificity,veracity,sensibility,adaptability,in-hibitor tolerance,consistency,balance,reaction condition verification,mixed samples,stability and inter batch consistency.The system efficiency of IDentifier DNA typing kit(YanHuang34)was compared with the PowerPlex® Fusion 6C System,VersaPlex® 27PY System and VeriFilerTM Plus PCR Amplifica-tion Kit.The IDentifier DNA typing kit(YanHuang34)was used to detect the swabs of biological samples in daily cases and the STR performances were observed.Results IDentifier DNA typing kit(YanHuang34)had good species specificity,veracity,adaptability,inhibitor tolerance and balance.The sensibility was up to 0.062 5 ng.It was able to detect different types of samples,degraded samples and inhibitor mixed samples.Complete DNA typing could be obtained for samples with the mixture ratio less than 4∶1.The system efficiency of IDentifier DNA typing kit(YanHuang34)was very high,with TDP up to 1-1.08×10-37,CPEtrio and CPEduo up to 1-5.47×10-14 and 1-6.43×10-9,respectively.For the touched biological samples in actual cases,the effective detection rate was 21.05%.The system efficiency of kinship,single parent and full sibling identifications was effectively improved.Conclusion The IDentifier DNA typing kit(YanHuang34)is adaptive to the GB/T 37226-2018 requirements.It can be used for individual identification and paternity identification,and is suitable for application in the field of forensic science.关键词
法医遗传学/短串联重复序列/IDentifier DNA分型盒(炎黄34)/性能验证/亲缘关系/个体识别Key words
forensic genetics/short tandem repeat(STR)/IDentifier DNA typing kit(YanHuang34)/efficiency validation/kinship/individual identification分类
医药卫生引用本文复制引用
高林林,谢炜,朱素娟,李达,王琴,洪亮,李佑英..IDentifier DNA分型盒(炎黄34)的法医学验证及应用评估[J].法医学杂志,2023,39(6):579-585,7.基金项目
杭州市农业与社会发展科研引导项目(20220919Y162) （20220919Y162）
