高等学校化学学报2024，Vol.45Issue(1)：90-99,10.DOI:10.7503/cjcu20230391
基于光诱导电子转移的比率型发光传感器定量检测生物样本中的多巴胺
A Ratiometric Luminescent Sensor Based on Photoinduced Electron Transfer for Quantitative Detection of Dopamine in Biological Samples
摘要
Abstract
The persistent luminescence particle material is a type of photoluminescent material that gradually emits stored energy in the form of light once excitation ceases.Owing to its exceptional optical properties,it finds extensive applications in biosensing and bioimaging.In this work,a ratiometric luminescent sensor(SEM-TYR)was developed by covalent coupling of dual-emission long-afterglow nanoparticles Sr2.992Mg0.9Si2O8:0.008Eu2+,0.10Mn2+(SEM)with exceptional luminescence performance and tyrosinase(TYR).This innovative design effectively eliminates spontaneous fluorescence interference and external disturbances,enabling highly sensitive and selective detection of dopamine(DA)in biological samples.When DA is present,tyrosinase facilitates its oxidation to dopaquinone,and in accordance with the principle of photoinduced electron transfer(PET),the photoactivated electron moiety of SEM-TYR is transferred to the electron acceptor dopaquinone,resulting in the attenuation of the 680 nm red afterglow observed in SEM-TYR.Under optimized conditions,the ratio Δ(I680/I460)showed a good linear relationship with the dopamine concentration in the range of 0.2-10 μmol/L and the limit of detection limit was 40 nmol/L.The developed method was applied for the detection of dopamine in human serum,urine and sweat after being verified by standard dopamine.Good agreement was achieved and the quantitative recovery was in the range of 94.8％—104.0％,91.3％—101.6％and 97.4％—105.2％,respectively.The RSD was less than 6.8％(n=10).The ratiometric lumines-cent sensor developed in this study has a potential application prospect in the determination of DA in biological samples.关键词
比率型发光传感器/多巴胺/长余辉纳米粒子/光诱导电子转移/无自发荧光Key words
Ratiometric luminescent sensor/Dopamine/Persistent luminescence nanoparticle/Photoinduced electron transfer/Autofluorescence-free分类
化学化工引用本文复制引用
朱润芝,王怡,纳佳雪,曹乐乐,张辉,王迎辉,孟哲..基于光诱导电子转移的比率型发光传感器定量检测生物样本中的多巴胺[J].高等学校化学学报,2024,45(1):90-99,10.基金项目
国家自然科学基金(批准号:21966024,21768003)、宁夏自然科学基金(批准号:2023AAC03018,2022AAC03045)和宁夏大学生创新创业训练项目(批准号:S202310749146)资助.Supported by the National Natural Science Foundation of China(Nos.21966024,21768003),the Natural Science Foundation of Ningxia,China(Nos.2023AAC03018,2022AAC03045),and the College Students'Innovation and Entrepreneurship Training Program of Ningxia,China(No.S202310749146). （批准号:21966024,21768003）
