基于光诱导电子转移的比率型发光传感器定量检测生物样本中的多巴胺OACSTPCD

The persistent luminescence particle material is a type of photoluminescent material that gradually emits stored energy in the form of light once excitation ceases.Owing to its exceptional optical properties,it finds extensive applications in biosensing and bioimaging.In this work,a ratiometric luminescent sensor(SEM-TYR)was developed by covalent coupling of dual-emission long-afterglow nanoparticles Sr2.992Mg0.9Si2O8:0.008Eu2+,0.10Mn2+(SEM)with exceptional luminescence performance and tyrosinase(TYR).This innovative design effectively eliminates spontaneous fluorescence interference and external disturbances,enabling highly sensitive and selective detection of dopamine(DA)in biological samples.When DA is present,tyrosinase facilitates its oxidation to dopaquinone,and in accordance with the principle of photoinduced electron transfer(PET),the photoactivated electron moiety of SEM-TYR is transferred to the electron acceptor dopaquinone,resulting in the attenuation of the 680 nm red afterglow observed in SEM-TYR.Under optimized conditions,the ratio Δ(I680/I460)showed a good linear relationship with the dopamine concentration in the range of 0.2-10 μmol/L and the limit of detection limit was 40 nmol/L.The developed method was applied for the detection of dopamine in human serum,urine and sweat after being verified by standard dopamine.Good agreement was achieved and the quantitative recovery was in the range of 94.8％—104.0％,91.3％—101.6％and 97.4％—105.2％,respectively.The RSD was less than 6.8％(n=10).The ratiometric lumines-cent sensor developed in this study has a potential application prospect in the determination of DA in biological samples.