高电压技术2023，Vol.49Issue(12)：5246-5259,14.DOI:10.13336/j.1003-6520.hve.20230196
协同脉冲增强不可逆电穿孔消融疗效的多参数效应分析及活体大动物实验研究
Multi-parameter Effect Analysis and Large Living Animal Experimental Study of Synergistic Pulse-enhanced Irreversible Electroporation Ablation
摘要
Abstract
The proposal of a new waveform of nano-microsecond synergistic pulsed electric field provides a promising research direction for enhancing the therapeutic efficacy of traditional irreversible electroporation ablation.However,the investigation of parameter effects in the new synergistic pulsed tumor ablation technology is still in its early stages.In this study,the Hepa1-6 liver cancer cell line was utilized as the research subject.From the level of individual cells and 2D simulated tissue,combining cell viability assessments and fluorescent molecular probe technology to measure the ablation range,we systematically analyzed the influences of various parameters of synergistic pulses(pulse width,voltage,number,frequency,and interval time)on tumor cell killing effects(ablation area and activity rate)for the first time.A synergistic parameter combination that significantly improved the therapeutic outcome was proposed.The results demonstrated that the inhibitory effect on tumor cells was positively correlated with nanosecond pulse width(300～800 ns),voltage(ns pulse:1.2～2 kV,μs pulse:200～400 V),and the number of synergistic pulses,while being negatively correlated with frequency(0.1～100 Hz).The effect of interval time(1 μs～1 ms)was found to be insignificant.Based upon these findings,the first preclinical large animal test was conducted to further validate the feasibility of synergistic pulse tissue ablation.This re-search demonstrates that synergistic pulses can significantly enhance the therapeutic efficacy of irreversible electroporation ablation,and a dose-effect relationship between pulse parameters and treatment outcomes can be estab-lished.The results can provide essential data support and experimental groundwork for the clinical application of the next-generation synergistic pulse irreversible electroporation tumor ablation technology.关键词
肿瘤治疗/不可逆电穿孔/协同脉冲/参数效应/临床前大动物试验Key words
tumor treatment/irreversible electroporation/synergistic pulse/parameter effect/preclinical large animal trial引用本文复制引用
岳亚琪,董守龙,马欣,彭文成,余亮,姚陈果..协同脉冲增强不可逆电穿孔消融疗效的多参数效应分析及活体大动物实验研究[J].高电压技术,2023,49(12):5246-5259,14.基金项目
国家自然科学基金(51807016 （）
52237010) （）
重庆市自然科学基金(cstc2020jcyj-msxmX0932) （cstc2020jcyj-msxmX0932）
重庆市高等教育教学改革研究项目(213052).Project supported by National Natural Science Foundation of China(51807016,52237010),Chongqing Natural Science Foundation of China(cstc2020jcyj-msxmX0932),Chongqing Higher Education Teaching Reform Research Project(213052). （213052）
