高电压技术2023，Vol.49Issue(12)：5292-5301,10.DOI:10.13336/j.1003-6520.hve.20220468
串内频率对高频纳秒脉冲串诱导A375黑色素瘤细胞电穿孔的影响
Effects of Intraburst Frequency on Electroporation of A375 Melanoma Cells Induced by High-frequency Nanosecond Pulse Bursts
摘要
Abstract
High-frequency nanosecond pulse bursts can effectively kill tumor cells,whereas,the killing mechanism,es-pecially the effect of the intraburst frequency on the induced electroporation,is still unclear.Taking A375 melanoma cells as the research object,we carried out in vitro cell experiments such as cell viability detection,short-term/long-term fluo-rescent dye detection,and cell micro-morphological detection to study the effect of the intraburst frequency of high-frequency nanosecond pulse bursts on cell membrane electroporation.The results of cell viability experiments show that high-frequency nanosecond pulse bursts can effectively kill cells,and the intraburst frequency has a threshold of 100 kHz that significantly reduces cell viability.The results of fluorescent dye experiments reveal that the decrease in cell via-bility is caused by irreversible electroporation,and there is a cumulative effect of intraburst frequency on electroporation.The results of scanning electron microscopy directly verify that the high-frequency nanosecond pulse bursts induce irre-versible electroporation of tumor cells.The experimental results in this paper provide an experimental basis for the mechanism of high-frequency nanosecond pulse bursts killing tumor cells.关键词
高频纳秒脉冲串/串内频率/A375黑色素瘤细胞/不可逆电穿孔/累积效应Key words
high-frequency nanosecond pulse bursts/intraburst frequency/A375 melanoma cells/irreversible electro-poration/cumulative effect引用本文复制引用
徐进,米彦,吴晓,李津津,唐均英..串内频率对高频纳秒脉冲串诱导A375黑色素瘤细胞电穿孔的影响[J].高电压技术,2023,49(12):5292-5301,10.基金项目
国家自然科学基金(52077022).Project supported by National Natural Science Foundation of China(52077022). （52077022）
