海南医学院学报2024，Vol.30Issue(1)：1-7,7.DOI:10.13210/j.cnki.jhmu.20231011.003
山茶油对H2O2诱导大鼠H9C2心肌细胞氧化应激损伤的保护作用
Protective effect of camellia oil on H2O2-induced oxidative stress injury in H9C2 cardiomyocytes of rats
摘要
Abstract
Objective:To investigate the protective effect of camellia oil on H2O2-induced oxidative stress injury in H9C2 cardiomyocytes.Methods:The survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2 was detected by CCK8 method.Normal cultured cells were treated with 200 μmol/L H2O2 for 24 h,and the oxidative stress injury model was established as the model group.Cells were pretreated with 1%,0.1%,0.01%concentration of camellia oil for 24 h,and H2O2 was added for 24 h as the experimental group.The changes of cell senescence,mitochondrial membrane potential,proliferation,apoptosis and migration in each group were observed by β-galactosidase senescence staining,mitochondrial membrane potential staining,EdU cell proliferation staining and scratch staining respectively.The activity of superoxide dismutase(SOD),lactate de-hydrogenase(LDH)and malondialdehyde(MDA)in each group were detected by the kit.Results:The viability H9C2 cardiomyo-cytes treated with different concentrations of H2O2 inhibited and positively correlated with H2O2 concentration(P<0.01).Com-pared with the blank control group,the positive rate of cell senescence,MDA content and LDH activity in the H2O2 model group were increased(P<0.01).Mitochondrial membrane potential,cell proliferation rate,mobility and SOD activity were decreased(P<0.01).Compared with the H2O2 model group,the senescence positive rate(P<0.01 or P<0.05),MDA content and LDH ac-tivity were decreased in the experimental group(P<0.01 or P<0.05).Mitochondrial membrane potential increased,cell prolifera-tion rate and mobility were increased(P<0.01 or P<0.05).Conclusion:Camellia oil can significantly inhibit the oxidative stress injury of H9c2 cells and play a protective role in cardiomyocytes.关键词
山茶油/H2O2/H9C2/氧化应激/保护Key words
Camellia oil/H2O2/H9C2/Oxidative stress/Protective分类
医药卫生引用本文复制引用
晏庆,郭蓁,孙赛男,黎婧,谭机永,黎静..山茶油对H2O2诱导大鼠H9C2心肌细胞氧化应激损伤的保护作用[J].海南医学院学报,2024,30(1):1-7,7.基金项目
This study was supported by the National Natural Science Foundation of China(82160597) （82160597）
Guangxi Natural Science Foundation Project(2020GXNSFAA159148) 国家自然科学基金委员会资助项目(82160597) （2020GXNSFAA159148）
广西自然科学基金项目(2020GXNSFAA159148) （2020GXNSFAA159148）
