海南医学院学报2024，Vol.30Issue(1)：8-14,7.DOI:10.13210/j.cnki.jhmu.20231030.002
二苯乙烯苷调控PI3K/AKT信号通路干预SH-SY5Y细胞凋亡的机制研究
Mechanism of stilbene glycosides on apoptosis of SH-SY5Y cells via regulating PI3K/AKT signaling pathway
摘要
Abstract
Objective:To investigate the effects of stilbene glycoside(TSG)on okadaic acid-induced apoptosis in human neuroblastoma cells(SH-SY5Y)via the PI3K/AKT pathway.Methods:The optimal concentration of OA was screened by CCK-8 assay,and SH-SY5Y cells were divided into the control group,the model group,the TSG group,the LY294002 group and the LY294002+TSG group.The proliferation and apoptosis in each group were detected by CCK-8 and TUNEL assays;Western blotting method and real-time fluorescence quantitative polymerase chain reaction were used to detect the expression of PI3K,P-PI3K(Y607),AKT,P-AKT(Ser473),Bcl-2 and Bax proteins.The relative protein expression was represented by P-PI3K(Y607)/PI3K,P-AKT(Ser473)/AKT and Bcl-2/Bax gray ratio.Results:CCK-8 screened the optimal concentration of OA as 40 nmol/L.Compared with the control group,the model group increased relative cell viability,decreased apoptosis rate,the pathway and apoptotic proteins expression levels of P-PI3K(Y607)/PI3K,P-AKT(Ser473)/AKT and Bcl-2/Bax were de-creased,the mRNA expression levels of PI3K,AKT and Bcl-2 were decreased,While Bax mRNA expression level increased(P<0.05);Compared with model group,the TSG group increased relative cell viability,decreased apoptosis rate,increased protein expression levels of P-PI3K(Y607)/PI3K,P-AKT(Ser473)/AKT,Bcl-2/Bax,and increased mRNA expression levels of PI3K,AKT,and Bcl-2,While Bax mRNA expression decreased(P<0.05),the LY294002 group decreased relative cell viabili-ty,increased apoptosis rate,P-PI3K(Y607)/PI3K protein expression levels were significantly decreased(P<0.05),P-AKT(Ser473)/AKT and Bcl-2/Bax protein expression levels were significantly decreased,but there was no statistical significance.PI3K,AKT and Bcl-2 mRNA expression levels were decreased,and Bax mRNA expression levels were increased(all P<0.05);Compared with the LY294002 group,the LY294002+TSG group increased relative cell viability,decreased apoptosis rate,and the protein expression levels of P-PI3K(Y607)/PI3K,P-AKT(Ser473)/AKT and Bcl-2/Bax were increased.The mRNA expression levels of PI3K,AKT,Bcl-2 were increased,and Bax was decreased(all P<0.05).Conclusion:Stilbene gly-coside may alleviate okadaic acid-induced apoptosis in SH-SY5Y cells by interfering with the PI3K/AKT signaling pathway,which in turn regulates the expression of apoptotic factors such as Bcl-2 and Bax.关键词
二苯乙烯苷/阿尔茨海默症/磷酸肌醇-3激酶抑制剂/磷酸肌醇3-激酶/蛋白激酶B/细胞增殖/细胞凋亡Key words
2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside/Alzheimer disease/LY294002/Phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/Cell proliferation/Apoptosis分类
医药卫生引用本文复制引用
康碧倩,朱晓莹,黄忠仕,李悦,何晓璇,肖振,胡睿,罗陈亮,乔明宇,吴桂有,李振中..二苯乙烯苷调控PI3K/AKT信号通路干预SH-SY5Y细胞凋亡的机制研究[J].海南医学院学报,2024,30(1):8-14,7.基金项目
This study was supported by the National Natural Science Foundation of China(81860709) （81860709）
Baise City Science and Technology Plan Project(Encyclopedia 20224139,Encyclopedia 20211807) （Encyclopedia 20224139,Encyclopedia 20211807）
2023 Youjiang Ethnic Medical College Graduate Innovation Program Project(YXCXJH2023013) 国家自然科学基金资助项目(81860709) （YXCXJH2023013）
百色市科技计划项目(百科20224139,百科20211807) （百科20224139,百科20211807）
2023年右江民族医学院研究生创新计划项目(YXCXJH2023013) （YXCXJH2023013）
