基于IL-6/JAK2/STAT3信号轴研究阳和平喘颗粒调控哮喘大鼠气道重塑作用机制OACSTPCD

Objective:To investigate the effects of Yanghe Pingchuan Granules on airway remodeling in asthmatic rats,and to explore the mechanism of Interleukin-6/Janus kinase 2/Signal transducing activator of transcription 3(IL-6/JAK2/STAT3)signal axis.Methods:We separated 42 healthy male SD rats into two groups,a control group(7)and a model group(35).The model group was sensitized with a combination of ovalbumin(OVA)and aluminum hydroxide for 2 weeks,while the control group was given an equal amount of physiological saline.After 2 weeks,the modeling group was randomly divided into Model group,Yanghe Pingchuan Granules high,medium and low dose groups and Dexamethasone group,each group consisted of 7 animals.After 4 weeks,OVA atomization and gavage were used for stimulation and treatment.Yanghe Pingchuan Granules high,middle and low groups were given 15.48,7.74,3.87g/kg Yanghe Pingchuan Granules daily,dexamethasone group was giv-en 0.062 5 mg/kg dexamethasone daily,and the other groups were given the same amount of normal saline.HE,PAS and Mas-son staining were used to observe the lung histopathological changes in rats.The levels of interleukin-6,IL-23 and IL-17A were detected by ELISA.The expression levels of JAK-2,P-JAK2,STAT3 and P-STAT3 in lung tissues were detected by Western blot.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the mRNA expression levels of IL-6,JAK2 and STAT3 in rat lung tissue.Results:The lung tissue structure of the model group was severely damaged compared to the con-trol group,accompanied by a great many of inflammatory cell infiltration,goblet cell hyperplasia,subepithelial collagen fiber depo-sition and airway epithelial thickening were more obvious.The expressions of IL-6,IL-23 and IL-17A in serum were significantly increased(P<0.01),the protein expression levels of JAK-2,P-JAK2,STAT3 and P-STAT3 and the mRNA expression levels of IL-6,JAK2 and STAT3 in lung tissue were significantly increased(P<0.01);Compared with the model group,inflammatory cell infiltration,goblet cell proliferation,subepithelial collagen fiber deposition and airway epithelial thickening were significantly reduced in each administration group,and the expressions of IL-6,IL-23 and IL-17A in serum were significantly decreased(P<0.01).The protein expression levels of JAK-2,P-JAK2,STAT3 and P-STAT3 and mRNA expression levels of IL-6,JAK2 and STAT3 in lung tissue were significantly decreased(P<0.01).Conclusion:Yanghe Pingchuan Granules can significantly alle-viate airway remodeling in asthmatic rats,and its mechanism may be through inhibiting the IL-6/JAK2/STAT3 signal axis.