胃饥饿素通过miR-455-5p靶向IGF-1R调控肝细胞胰岛素敏感性的作用及机制研究OACSTPCD
Ghrelin regulates insulin resistance by targeting insulin-like growth factor-1 receptor via miR-455-5p in hepatic cells
目的:探究胃饥饿素通过调控miR-455-5p影响肝细胞胰岛素敏感性的作用机制.方法:采用高糖构建HepG2细胞胰岛素抵抗模型,造模成功后使用去酰基化胃饥饿素(DAG,1 μmol/L)干预,分别转染miR-455-5p 模拟物(miR-455-5p mimic)或对照物(NC mimic).检测各组细胞葡萄糖消耗量和细胞内糖原含量.采用荧光原位杂交分析HepG2细胞内miR-455-5p表达水平.应用生物信息学分析、荧光素酶报告基因实验来鉴定与…查看全部>>
Objective:To explore the mechanism by which ghrelin regulates insulin sensitivity through modulation of miR-455-5p in hepatic cells.Methods:HepG2 cells were treated with or without Ghrelin(DAG)(1 μmol/L).Glucose consump-tion,intracellular glycogen content,phosphorylation of PI3K and Akt stimulated by insulin,expression of miR-455-5p,as well as IGF-1R protein level were analyzed.In addition,bioinformatic analysis,dual luciferase reporter assay,miR-455-5p mimi…查看全部>>
郭展宏;居悦俊;沈婷;张琳琪;盛忠奇;吴润泽;孔颖宏
江苏省常熟市第二人民医院内分泌科,江苏 常熟 215500江苏省常熟市第二人民医院内分泌科,江苏 常熟 215500江苏省常熟市第二人民医院内分泌科,江苏 常熟 215500江苏省常熟市第二人民医院内分泌科,江苏 常熟 215500江苏省常熟市第二人民医院内分泌科,江苏 常熟 215500江苏省常熟市第二人民医院内分泌科,江苏 常熟 215500江苏省常熟市第二人民医院内分泌科,江苏 常熟 215500
临床医学
胃饥饿素miR-455-5pIGF-1R胰岛素抵抗HepG2细胞
GhrelinmiR-455-5pIGF-1RInsulin resistanceHepG2 cells
《海南医学院学报》 2024 (1)
21-28,8
This study was supported by Changshu Science and Technology Plan(Social Development)Project(CS202130)Key Project of Changshu No.2 People's Hospital(CSEY2021007) 常熟市科技计划(社会发展)项目(CS202130)常熟市第二人民医院重点项目(CSEY2021007)
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