海南医学院学报2024，Vol.30Issue(1)：21-28,8.DOI:10.13210/j.cnki.jhmu.20230906.002
胃饥饿素通过miR-455-5p靶向IGF-1R调控肝细胞胰岛素敏感性的作用及机制研究
Ghrelin regulates insulin resistance by targeting insulin-like growth factor-1 receptor via miR-455-5p in hepatic cells
摘要
Abstract
Objective:To explore the mechanism by which ghrelin regulates insulin sensitivity through modulation of miR-455-5p in hepatic cells.Methods:HepG2 cells were treated with or without Ghrelin(DAG)(1 μmol/L).Glucose consump-tion,intracellular glycogen content,phosphorylation of PI3K and Akt stimulated by insulin,expression of miR-455-5p,as well as IGF-1R protein level were analyzed.In addition,bioinformatic analysis,dual luciferase reporter assay,miR-455-5p mimic or in-hibitor treatment were conducted to investigate the molecular mechanisms.Results:High glucose treatment upregulated miR-455-5p expression but reduced glucose consumption and glycogen content.DAG reversed the effect of high glucose on glu-cose metabolism,increased protein level of IGF-1R and phosphorylation of PI3K/Akt stimulated by insulin,as well as downregu-lated miR-455-5p expression.Bioinformatic analysis indicated IGF-1R was the target of miR-455-5p.Dual luciferase reporter as-say,as well as transfection with miR-455-5p mimic/inhibitor confirmed that DAG activated the IGF-1R/PI3K/Akt signaling via inhibiting miR-455-5p.Conclusion:DAG improves insulin resistance via miR-455-5p-mediated activation of the IGF-1R/PI3K/Akt signaling,suggesting that suppression of miR-455-5p or activation of DAG may be potential targets for T2DM therapy.关键词
胃饥饿素/miR-455-5p/IGF-1R/胰岛素抵抗/HepG2细胞Key words
Ghrelin/miR-455-5p/IGF-1R/Insulin resistance/HepG2 cells分类
医药卫生引用本文复制引用
郭展宏,居悦俊,沈婷,张琳琪,盛忠奇,吴润泽,孔颖宏..胃饥饿素通过miR-455-5p靶向IGF-1R调控肝细胞胰岛素敏感性的作用及机制研究[J].海南医学院学报,2024,30(1):21-28,8.基金项目
This study was supported by Changshu Science and Technology Plan(Social Development)Project(CS202130) （Social Development）
Key Project of Changshu No.2 People's Hospital(CSEY2021007) 常熟市科技计划(社会发展)项目(CS202130) （CSEY2021007）
常熟市第二人民医院重点项目(CSEY2021007) （CSEY2021007）
