海南医学院学报2024，Vol.30Issue(1)：29-38,10.DOI:10.13210/j.cnki.jhmu.20230927.002
苓桂术甘汤干预非酒精性脂肪肝病的药效学研究及作用机制探讨
Pharmacodynamic study and mechanism of action of Linggui Zhugan Decoction in the intervention of Nonalcoholic fatty liver disease
摘要
Abstract
Objective:To explore the therapeutic effect of Linggui Zhugan Decoction on nonalcoholic fatty liver disease through in vivo animal experiments,and to explore the mechanism of action of Linggui Zhugan Decoction in the intervention of nonalcoholic fatty liver disease through network pharmacology and molecular docking technology.Methods:A rat model of non-alcoholic fatty liver disease was established after 20 weeks of high-fat feeding,and the rats were divided into six groups,nor-mal group,model group,simvastatin group(4 mg/kg),low-dose group of Linggui Zhugan Decoction(2.1 g/kg),medium-dose group of Linggui Zhugan Decoction(4.2 g/kg),and high-dose group of Linggui Zhugan Decoction(8.4 g/kg),and the liver mor-phological changes of HE staining and oil red O staining after the intervention of Linggui Zhugan Decoction were observed,and the microplate reader detected aspartate aminotransferase,alanine aminotransferase,Four levels of blood lipids.Network pharma-cology combined with molecular docking was used to screen the potential mechanism and target of Linggui Zhugan Decoction for the intervention of non-alcoholic fatty liver disease.Results:Linggui Zhugan Decoction significantly improved the levels of alanine aminotransferase and alanine aminotransferase in model rats(P<0.05).HE staining and oil red O staining showed that Linggui Zhugan Decoction significantly reduced liver lipid deposition in rats in the model group.Linggui Zhugan Decoction could signifi-cantly reduce the quadruplical levels of blood lipids in model rats(P<0.05).Network pharmacology screened out 10 key targets and 5 key signaling pathways;The molecular docking results showed that the first 20 active ingredients all had good binding ability to the top 10 targets.Conclusion:Linggui Zhugan Decoction has the effect of regulating blood lipids and improving liver tissue le-sions in non-alcoholic fatty liver disease,and preliminarily verifies the regulatory effect of Linggui Zhugan Decoction on lipid metabolism-related genes.关键词
网络药理学/分子对接/苓桂术甘汤/非酒精性脂肪肝Key words
Network pharmacology/Molecular docking/Linggui Zhugan Decoction/Nonalcoholic fatty liver disease分类
医药卫生引用本文复制引用
赵传志,丁慧敏,胡紫晴,都永勤,周兰,周鹏,王靓..苓桂术甘汤干预非酒精性脂肪肝病的药效学研究及作用机制探讨[J].海南医学院学报,2024,30(1):29-38,10.基金项目
Anhui Natural Science Foundation General Project(2208085MH277) 安徽自然科学基金面上项目(2208085MH277) （2208085MH277）
