天龙竭胶囊对肺纤维化模型大鼠α-SMA和E-Cadherin的影响OACSTPCD

Objective:To observe the effect of Tianlongjie Capsule on bleomycin induced pulmonary fibrosis(PF)in ratsα-The impact of SMA/E cadherin and the mechanism by which Tianlongjie Capsule improves PF are explored.Method:Sixty Wistar rats were randomly di-vided into a blank group,a model group,a low-dose group of Tianlongjie Capsule(0.51 g·kg-1),a medium dose group of Tianlongjie Capsule(1.01 g·kg-1),a high-dose group of Tianlongjie Capsule(2.02 g·kg-1),and a pirfenidone group(0.12 mg·kg-1).Except for the blank group,rats in all other groups were given tracheal instillation of bleomycin(5 mg·kg-1)to replicate pulmonary fibrosis models.After 14 days of modeling,each group of rats was given corresponding drug intervention with gavage.The blank group and model group were given equal volume of physiological saline with gavage once a day for 28 consecutive days.Take blood from the abdominal aorta of rats for blood gas analysis.A reagent kit was used to detect the content of hydroxyproline(HYP)in lung tissue.Mas-son staining and HE staining were used to observe pathological changes in rat lung tissue.RT-PCR and Western blot was used to de-tect the expression levels of α-Alpha smooth muscle actin,α-SMA,E-cadherin,collagen Ⅰ(COL-Ⅰ),COL-Ⅲ protein and mRNA in the lung tissue.Results:Compared with the blank group,the lung index and lung tissue HYP level of the model group rats were significantly increased(P＜0.01),while partial pressure of oxygen(PO2)and oxygen saturation(SO2)were significantly re-duced(P＜0.01).The expression levels of COL-Ⅰ,COL-Ⅲ,and α-SMA as well as E-Cadherin protein and mRNA were signif-icantly upregulated(P＜0.05);Compared with the model group,Tianlongjie Capsule can significantly reduce lung index and lung tis-sue HYP levels in rats(P＜0.05),significantly increase PO2 and SO2(P＜0.05),and significantly decrease the expression levels of lung tissue COL-Ⅰ and COL-Ⅲα-SMA,E-Cadherin protein and mRNA significantly reduce(P＜0.05).HE staining and Mas-son staining suggested that in the model group of rats,the alveolar structure was damaged,a large number of inflammatory cells infiltra-ted,the lung interstitium expanded,the alveolar septa thickened and the collagen deposition and fiber deposition in the lung tissue sig-nificantly increased,forming fibrotic lesions.Tianlongjie Capsule can significantly improve lung tissue lesions in model rats,with high-dose effects being the most significant.Conclusion:Tianlongjing can significantly improve pathological damage,lung function,and pul-monary fibrosis in PF rats,and its effect may be related to the regulation of the expression of cadherin-αSMA/E and their involvement in epithelial mesenchymal transition.