凉血解毒活血方治疗大鼠肾毒血清肾炎新月体病变OACSTPCD

Objective:To characterize the efficacy of Liangxue Jiedu Huoxue Fang(LJH)in treating crescentic lesions in rats with neph-rotoxic serum nephritis(NTSN).Method:45 Wistar Kyoto rats were randomly divided into a normal group,Model Ⅰ group(taken on the 7th day of modeling),Model Ⅱ group,methylprednisolone group,and LJH + methylprednisolone group,with 9 rats in each group.The method for establishing a rat NTSN model is to extract the glomerular basement membrane(GBM)antigen from Sprague Dawley rats and perform symmetrical multi-point subcutaneous injection on the back of New Zealand white rabbits to prepare nephrotoxic ser-um.After 7 days of pre immunization in WKY rats,renal toxic serum was injected into the tail vein continuously for 3 days.Except for Model Ⅰ group,all other groups started gastric lavage on the 7th day of modeling,lasting for 7 days,and urine was collected for 24 hours after the last gastric lavage.Apply PeriodicAcid Schiff(PAS)staining to observe the pathological changes in renal tissue of rats in each group,including the percentage of crescents.Use a fully automated biochemical analyzer to detect the 24-hour urine protein content,urine immunoglobulin G(IgG)and creatinine(Cr)levels in each group of rats.Detection of integrin in renal tissue of rats in each group using immunohistochemical method β Localization and expression level of integrinbeta-2(ITGB2)protein.Results:The glomeruli and surrounding tubular cells of the normal group rats were arranged neatly,with normal morphology and clear boundaries,and no significant damage was observed;On the 7th day of modeling in Model GroupⅠrats,25%to 50%of the glomeruli formed cellular crescents,and fibrin deposition was visible in the crescents;On the 14th day of modeling,a large number of cellular crescentic lesions appeared in the glomeruli of ModelⅡrats,with a crescentic body proportion of≥50%.Compared with the normal group,the 24-hour urine protein quantification and crescent percentage in Model Ⅰ group were significantly increased(P＜0.05);Compared with ModelⅠ group,Model Ⅱ group showed a significant increase in 24-hour urine protein quantification and crescent percentage(P＜0.05).Compared with the normal group,the 24-hour urine protein content,crescent percentage,and urine IgG/Cr ratio of ModelⅡgroup rats were significantly increased(P＜0.05);Compared with ModelⅡgroup,the 24-hour urinary protein content,crescent percentage,and urinary IgG/Cr ratio of rats in the methylprednisolone group and LJH + methylprednisolone group were significantly reduced(P＜0.05);Compared with the methylprednisolone group,the 24-hour urine protein content,crescent percentage,and urine IgG/Cr ratio of the LJH +methylprednisolone group rats were significantly reduced(P＜0.05).The immunohistochemical results showed that the IT-GB2 protein was almost not expressed in the glomeruli of the normal group rats,while the expression of ITGB2 protein was observed in the glomerular crescent cells and parietal epithelial cells of the Model Ⅱ group rats;Compared with Model Ⅱ group,the expression of ITGB2 in the glomeruli of rats in the methylprednisolone group and LJH + methylprednisolone group was significantly reduced(P＜0.05);Compared with the methylprednisolone group,the expression of ITGB2 in the glomeruli of rats in the LJH +methylprednisolone group was significantly reduced(P＜0.05).Conclusion:LJH attenuates crescentic lesions and reduces urinary protein levels in rats with NTSN,which may be related to the reduction of ITGB2 protein expression level.