华南农业大学学报2024，Vol.45Issue(2)：199-206,8.DOI:10.7671/j.issn.1001-411X.202307007
水稻抗白叶枯病种质资源筛选及抗性基因鉴定
Germplasm resource screening of resistance against bacterial blight and identification of resistance gene in rice
摘要
Abstract
[Objective]The purpose is to screen rice varieties with resistance to the Ⅴ and Ⅸ pathotypes of Xanthomonas oryzae pv.oryzae(Xoo).[Method]Ninety-seven brown planthopper resistant rice varieties collected from tropical and subtropical areas were surveyed against Ⅴ and Ⅸ types of Xoo.The leaf-cutting inoculation method was used to identify the resistance level of rice varieties to bacterial blight at the seedling and adult stages.A mapping population was developed for a variety with excellent resistance performance,which was applied to map and clone the resistance genes.[Result]Twenty and eight rice varieties were detected to be resistant to Ⅴ type at the seedling and adult stages respectively,and four varieties showed moderate or high resistance at both stages.Moreover,thirty-four and nine rice varieties were identified to be resistant to Ⅸ type at the seedling and adult stages respectively,and one variety showed moderate or high resistant level at both stages.Rice variety'BX02'showed excellent resistance to Ⅴ type of Xoo at both stages.Therefore,one F2 mapping population was developed by crossing'BX02'with'9311',and the resistance locus was identified on chromosome 5 with the logarithm of adds(LOD)score of 40.6.This mapping interval contained the cloned xa5 gene with resistance to Ⅴ type of Xoo.Sequence amplification analysis confirmed that it was the bacterial blight resistance gene xa5.[Conclusion]Four and one varieties were detected to be resistant to Ⅴ and Ⅸ types of Xoo at both stages respectively,which provides materials for rice resistance breeding against the bacterial blight.关键词
稻种资源/水稻白叶枯病/基因定位/xa5/抗性基因Key words
Rice germplasm/Rice bacterial blight/Gene mapping/xa5/Resistance gene分类
农业科技引用本文复制引用
钟小惠,蒋哲,陆守腾,黄福钢,王彩先,农保选,邱永福..水稻抗白叶枯病种质资源筛选及抗性基因鉴定[J].华南农业大学学报,2024,45(2):199-206,8.基金项目
中央引导地方科技发展资金专项(桂科ZY21195040) （桂科ZY21195040）
广西创新驱动发展专项(桂科-AA22068087-3) （桂科-AA22068087-3）
