华南农业大学学报2024，Vol.45Issue(2)：256-265,10.DOI:10.7671/j.issn.1001-411X.202308003
香蕉抗枯萎病株系内生菌的分离鉴定及抑菌促生效果
Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects
摘要
Abstract
[Objective]To screen and identify endophytic bacteria that can inhibit Fusarium oxysporum f.sp.cubense(Foc)isolated from roots,bulbs and pseudostems of wilt-resistant strains of Williams banana,and study their inhibitory and growth-promoting effects.[Method]Through 16S rRNA gene sequence and physiological and biochemical tests,species identification and phylogenetic analysis of endophytic bacteria in banana fusarium wilt resistant strains were carried out.The plate confrontation test was carried out to detect the antibacterial ability of strain and explore the types of antibacterial related active substances secreted by antagonist.The biocontrol and growth-promoting functions of endophytic bacteria in banana resistant strains to wilt disease were analyzed by pot experiment.The endophytes with good biocontrol and growth promoting effects were selected and verified in field.[Result]Thirteen representative strains were isolated,among which,there were 8 strains with antibacterial activity against Foc 1 and 7 strains with antibacterial activity against Foc 4.Three biocontrol strains with high inhibitory activity and growth promotion ability were selected by the comprehensive results of biocontrol and growth promoting effects in pot and field experiments,including Bacillus velezensis XG1028,Klebsiella mutans XQJ0301 and Bacillus siamensis XG0103.[Conclusion]Three screened strains can enrich the germplasm resources of microorganisms resistant to banana wilt disease,and provide a reference for finding the new safe and environmentally friendly biological control agents controlling banana wilt disease.关键词
香蕉枯萎病/拮抗菌/拮抗因子/生防功能/促生效果Key words
Banana wilt/Antagonistic bacteria/Antagonistic factor/Biocontrol function/Growth promoting effect分类
农业科技引用本文复制引用
李锦,谭德东,邱济平,杨昌胜,许刚,谭志远..香蕉抗枯萎病株系内生菌的分离鉴定及抑菌促生效果[J].华南农业大学学报,2024,45(2):256-265,10.基金项目
国家自然科学基金(31970001) （31970001）
