华侨大学学报（自然科学版）2024，Vol.45Issue(1)：35-46,12.DOI:10.11830/ISSN.1000-5013.202311012
一株多药耐药Comamonas kerstersii细菌的基因组分析
Genomic Insights of A Multi-Drug Resistant Comamonas kerstersii Isolate
摘要
Abstract
A multi-drug resistant C.kerstersii 121606 was isolated from a patient with a urinary tract infec-tion,and the antimicrobial susceptibility testing(AST)and whole genome sequencing were performed,then comparative genomic analyses were conducted with seven representative Comamonas species strains and Acidovorax species by average nucleotide identity(ANI)analysis using OrthoANI and single nucleotide poly-morphism(SNP)analysis by snpTree web server.Finally,genomic sequence annotation was conducted with RAST server,as well as functional annotation of orthologous clusters by OrthoVenn,prediction of antibiotic resistance genes(ARGs)by CARD,CRISPRs by CRISPR recognition tool,and prophages by PH AST.These data revealed that C.kerstersii 121606 was a multi-drug resistant bacterium with similar genetic components to other seven Comamonas species and Acidovorax species.The presence of ARGs in its genome helped to ex-plain its multi-drug resistance mechanisms.These findings provide valuable insights for the study of novel an-tibiotics to manage multi-drug resistant C.kerstersii infections.关键词
Comamonas kerstersii/多药耐药性/比较基因组分析/抗生素耐药性基因/CRISPR/前噬菌体Key words
Comamonas kerstersii/multi-drug resistance/comparative genomic analysis/antibiotic resistance gene/CRISPR/prophage分类
生物科学引用本文复制引用
王慧,明德松,王明席..一株多药耐药Comamonas kerstersii细菌的基因组分析[J].华侨大学学报（自然科学版）,2024,45(1):35-46,12.基金项目
福建省厦门南方海洋研究中心科研资金资助项目(14GYY008NF08) （14GYY008NF08）
方树福堂基金会和方润华基金会资助项目(14X30127) （14X30127）
