湖南农业大学学报（自然科学版）2023，Vol.49Issue(6)：631-639,9.DOI:10.13331/j.cnki.jhau.2023.06.001
油菜芥甘种间杂交后代F2及BC1F1群体的遗传分析
Genetic analysis of F2 and BC1F1 progenies from the interspecific cross between Brassica juncea and Brassica napus
摘要
Abstract
The primers were designed by use of the sequences of chromosome-specific genes in Brassica juncea and B.napus as template and genotyping was performed to trace the transmission of chromosomes in the F2 and BC1F1 populations of B.juncea×B.napus hybrids.The results showed that the chromosomes An03,An08,An09,An10,Cn03 and Cn09 from B.napus and Aj03 and Aj10 from B.juncea were not lost in the BC1F1 population,while loss frequency of the chromosome Bj07 was the highest,62.83%.In the F2 population,the Aj-and Bj-genome chromosomes from B.juncea were lost more frequently than the An-and Cn-genome from B.napus,and the chromosomes An08 and Cn03from B.napus and Bj08 from B.juncea were lost at frequency of 1.36%,1.36%and 0.90%,respectively.However,the chromosome Bj07 was lost at 56.56%.For the BC1F1 progeny,with the mean seed setting of 0.60,up to 5.31 under self-pollination,and 1.95,up to 12.85 under open pollination.For the F2 progeny the mean seed setting of 0.66,up to 17.70 under self-pollination,and 2.18,up to 23.05 under open pollination.These results provided a theoretical basis for breeding by interspecific cross of B.juncea and B.napus,which would help enhance germplasm of B.napus by introducing elite genes of B.juncea.关键词
油菜/芥甘杂交/基因标记/染色体/花粉可染率/结实率Key words
rape/interspecific cross between Brassica juncea and B.napus/gene marker/chromosome/pollen stainability/seed setting分类
农业科学引用本文复制引用
杨淑婕,郭诗芬,彭雅雯,刘忠松..油菜芥甘种间杂交后代F2及BC1F1群体的遗传分析[J].湖南农业大学学报（自然科学版）,2023,49(6):631-639,9.基金项目
国家自然科学基金项目(U20A2029、31571712) （U20A2029、31571712）
