湖南农业大学学报（自然科学版）2023，Vol.49Issue(6)：652-660,9.DOI:10.13331/j.cnki.jhau.2023.06.004
灭菌方式对植烟土壤微生物群落的影响
Effects of soil disinfestation methods on microbial community in tobacco planting fields
摘要
Abstract
Tobacco planting soil with severe continuous cropping obstacles were treated by physical fumigation,chemical fumigation and reductive soil disinfestation based on sugarcane bagasse,with untreated soil as the control.Then,the changes in the soil physicochemical properties and microbial communities were analyzed,and FUNGuild platform was applied for functional prediction of fungal communities.Results showed that compared with the control,chemical fumigation significantly increased the content of hydrolyzed nitrogen in the soil,physical fumigation significantly increased the content of available phosphorus and available potassium,and reductive soil disinfestation significantly increased the pH value and organic matter content of the soil.The number of Fusarium oxysporum in the soil treated with physical fumigation,chemical fumigation,and reductive soil disinfestation decreased by 26.74%,41.39%and 38.46%,respectively;these sterilization methods all had inhibitory effects on Fusarium oxysporum in tobacco planting fields.The composition of microbial communities in soil changed significantly with different disinfestation methods.Chemical fumigation and reductive soil disinfestation increased the relative abundance of Bacillus,Tuberibacillus,Clostridium,and Bacteroides in the soil.In reductive soil disinfestation treated soil,Hydrogenospora,Ruminiclostridium and Bacteroides are the dominant bacterial genera.Function prediction for microbial community shows that physical fumigation leads to an increase in pathotrophic fungi in the soil,chemical fumigation transforms nutritional type of fungi from saprotroph-symbiotroph to pathotroph-symbiotroph,and reductive soil disinfestation significantly reduces the abundance of pathotrophic fungi and promotes the large-scale reproduction of saprophytic fungi.关键词
植烟土壤/土壤强还原/物理熏蒸/化学熏蒸/微生物群落/功能预测Key words
tobacco planting soil/reductive soil disinfestation/physical fumigation/chemical fumigation/microbial community/functional prediction分类
农业科学引用本文复制引用
魏光钰,胡勇,吴永琴,张清壮,李鑫..灭菌方式对植烟土壤微生物群落的影响[J].湖南农业大学学报（自然科学版）,2023,49(6):652-660,9.基金项目
贵州省烟草公司贵阳市公司科技项目(筑烟科[2018]4号) （筑烟科[2018]4号）
