湖南农业大学学报（自然科学版）2023，Vol.49Issue(6)：684-688,5.DOI:10.13331/j.cnki.jhau.2023.06.008
根干反向生长模拟对厚朴皮总酚含量的诱导及代谢组学分析
Induction of total phenol content and metabolomics analysis of Magnolia officinalis errata bark by root trunk reverse growth simulation
摘要
Abstract
Total phenols were determined by high performance liquid chromatography(HPLC)and the metabolomics were analyzed by LC-MS technique for the experimental material,Magnolia officinalis The studied sample did exposed root and embedded trunk treatments with the root bark and trunk bark as the control.The results showed that the total phenolic content of trunk bark and root bark increased in both the embedded trunk and exposed root treatments.107 of 873 detected metabolites in Magnolia officinalis,were significantly different between the control root bark and control trunk bark group;71 metabolites were significantly different between the exposed root bark and control trunk bark group,and 76 metabolites were significantly different between the embedded trunk bark and control root bark group.These differential metabolites among the treatment groups were mainly up-regulated.The simulated treatment of root trunk reverse growth caused significant differences in lipids and lipid compounds,organic acids and their derivatives,organic oxides,lignans,neolignans and their related compounds;and the differential metabolites were mainly clustered in β-alanine metabolism,histidine metabolism and other pathways.The root trunk reverse growth simulation treatment induced a significant decrease in the amount of differential metabolites between the treated and simulated sites,and caused a positive response of amino acids and metabolic pathways.关键词
厚朴/根干反向生长模拟/总酚/差异代谢物Key words
Magnolia officinalis/root-trunk reverse growth simulation/total phenol/differential metabolites分类
中医学引用本文复制引用
杜甜甜,汪明磊,陈阳峰,朱妙华,肖深根..根干反向生长模拟对厚朴皮总酚含量的诱导及代谢组学分析[J].湖南农业大学学报（自然科学版）,2023,49(6):684-688,5.基金项目
湖南省创新性自然科学基金项目(2021JJ30346) （2021JJ30346）
湖南省中药材产业技术体系(湘财农指[2023]66号) （湘财农指[2023]66号）
湖南省创新型省份建设专项(2021SK2012) （2021SK2012）
