湖南农业大学学报（自然科学版）2023，Vol.49Issue(6)：689-693,736,6.DOI:10.13331/j.cnki.jhau.2023.06.009
蘘荷叶枯病病原菌的分离鉴定与生物学特性
Isolation,identification and biological characteristics of the pathogen causing leaf blight of Zingiber mioga
摘要
Abstract
Diseased leaves from Zingiber mioga with leaf blight were collected from severely affected fields,and pathogenic bacteria were isolated and purified using tissue isolation method.The representative pathogenic strain RH-1 was obtained,further identification of strain RH-1 was conducted and biological characteristics of the strain were determined.Combining the results of morphological characteristics,molecular and biological identification,constructed fungal internal transcriptional spacer regions(ITS),phylogenetic tree based on β-Tubulin and TEF-1α,the pathogen was identified as Fusarium oxysporum.Strain RH-1 was cultured on PDA medium with different temperatures(5,10,15,20,25,30,35℃),pHs(4,5,6,7,8,9,10),and light durations(continuous light,continuous darkness,12 hours of light/12 hours of darkness,and 24 hours of light/24 hours of darkness).The results showed that the mycelium grew faster under 30℃,pH9,and continuous light conditions.Using glucose α-lactose and maltose as carbon sources,and yeast extract,beef extract,(NH4)2SO4,NH4Cl,peptone,and NH2CH2COOH as nitrogen sources to replace the carbon and nitrogen sources in Czapek basic culture medium in equal amounts.mycelium growth showed that the optimal carbon source was glucose or maltose,and the optimal nitrogen source was beef extract or peptone.Strain RH-1 was inoculated into red and green Zingiber mioga respectively,and it was found that green Zingiber mioga has stronger disease resistance.关键词
蘘荷/病原菌/尖孢镰刀菌/生物学特性Key words
Zingiber mioga/pathogen/Fusarium oxysporum/biological characteristics分类
植物保护学引用本文复制引用
王小秋,葛礼姣,仇亮,翟彩娇,王广达,宋益民,程玉静..蘘荷叶枯病病原菌的分离鉴定与生物学特性[J].湖南农业大学学报（自然科学版）,2023,49(6):689-693,736,6.基金项目
南通市科技局科技计划项目(JC2021019) （JC2021019）
江苏省蘘荷种质资源圃项目(通农财[2022]31号) （通农财[2022]31号）
江苏沿江地区农业科学研究所青年基金(YJ[2021]005) （YJ[2021]005）
