湖南农业大学学报（自然科学版）2023，Vol.49Issue(6)：723-729,7.DOI:10.13331/j.cnki.jhau.2023.06.014
中国七省(区)牛蜱的种类鉴定及其携带无形体的分子检测
Identification of tick species in cattle and detection of Anaplasma spp.in these ticks in 7 provinces and regions of China
摘要
Abstract
To identify the species of ticks in cattle and to investigate the Anaplasma spp.in ticks,572 ticks were collected from 7 provinces and regions(Henan,Hunan,Hainan,Sichuan,Guizhou,Guangdong and Guangxi)of China.Morphological and molecular biological methods were used to identify the species of these ticks,and PCR was conducted to analyze Anaplasma infection in these ticks.Sequence analysis showed that 572 ticks were identified as Rhipicephalus microplus(R.microplus),and three clades(A,B and C)of R.microplus were found.the sequence of 16S rRNA gene of Anaplasmas amplified from ticks showed R.microplus carried the Anaplasmas.The prevalence of Anaplasmas in ticks was 38.8%(222/572),and the prevalence of Anaplasmas marginale,Anaplasmas capra,Anaplasmas platys and Anaplasmas phagocytophilum were 23.6%(135/572),7.3%(15/572),5.3%(42/572),2.6%(30/572),respectively.Co-infection of Anaplasma species was only found in ticks from Hunan Province.This is the first report of R.microplus clade C in China,and Anaplasma margina is the common Anaplasma species.关键词
牛蜱/微小扇头蜱/物种鉴定/无形体/中国7省(区)Key words
cattle ticks/Rhipicephalus microplus/species identification/Anaplasma/7 provinces and regions in China分类
农业科技引用本文复制引用
潘海钰,张雪玲,麻林坤,马平平,李容,吴雅丽,邓园萍,刘国华..中国七省(区)牛蜱的种类鉴定及其携带无形体的分子检测[J].湖南农业大学学报（自然科学版）,2023,49(6):723-729,7.基金项目
国家自然科学基金面上项目(32172884) （32172884）
湖南省教育厅重点项目(22A0148) （22A0148）
