渔业科学进展2024，Vol.45Issue(1)：105-117,13.DOI:10.19663/j.issn2095-9869.20220901003
凡纳滨对虾类胰岛素肽基因的结构及表达分析
Gene Structure and Expression Analysis of Insulin-Like Peptide in the Pacific White Shrimp Litopenaeus vannamei
摘要
Abstract
Insulin-like peptide(ILP)is a member of the insulin superfamily with evolutionary conservation and is one of the most important factors affecting animal life activities.In this study,a full-length of ILP1 gene in Pacific white shrimp,Litopenaeus vannamei,was cloned,and the mRNA length consisted of 812 bp with an open reading frame(ORF)of 543 bp,encoding 180 amino acids.Sequence analysis showed that the predicted molecular weight of LvILP1 protein was 20.81 kDa,and the theoretical isoelectric point was 9.45 and the instability coefficient was 96.20.There was a signal peptide,no transmembrane structure.It was deduced that it was located outside the cell and is an alkaline unstable secreted protein.Structure prediction found that LvILPl protein had the conserved I1GF domain of the insulin superfamily,which was composed of the N-terminal signal peptide,B-chain,C-peptide and A-chain,as well as six conserved cysteine sites and two cleavage sites.Phylogenetic analysis found that LvILPl was most closely related to ILP7 in Penaeus monodon,and clustered with ILP1 of Crustaceans to form a branch,and then clustered with ILP7 of Invertebrate,Relaxin,Insulin and Insulin-like growth factor(IGF)of vertebrate;ILP7 of Invertebrate was evolutionarily closest to outgroup sea anemone ILP1,suggesting that it may be more similar to the ancestor gene of the insulin superfamily.Transcription factor prediction found that the possible transcription factors of LvILP1 are Forkhead box protein O3(FoxO3),Glucocorticoid receptor(GR),CAAT region/enhancer binding protein(C/EBP)and Signal transduction and transcription activator protein(STAT);The protein interaction analysis found that LvILP1 interacted with Insulin receptor(IR)on the cell membrane,nerve signaling molecules(VGLUT1,SYT 1_3),Glycoprotein hormone beta 5(GPHB5),Bursicon alpha,etc.By analyzing the biological function analysis of these transcription factors and interacting proteins,it is speculated that LvILP1 may play an important role in regulating shrimp growth and development,response to hormonal stimulation,nervous system homeostasis,carbohydrate homeostasis,postmolt tissue remodeling and reproductive development.The analysis showed that LvILP1 was expressed in the early developmental stage of shrimp,and expressed in all tissues of adults,but the expression level in the eye stalk was the highest.This study provides important information for in-depth understanding of the structure,evolution,function and expression of ILP in shrimp,as well as clues for molecular breeding and healthy farming of shrimp.关键词
凡纳滨对虾/类胰岛素肽/基因结构/功能预测/表达分析Key words
Litopenaeus vannamei/Insulin-like peptide/Gene structure/Functional prediction/Expression analysis分类
生物科学引用本文复制引用
苏曼文,张晓军,袁剑波,张小溪,杨铭,李富花..凡纳滨对虾类胰岛素肽基因的结构及表达分析[J].渔业科学进展,2024,45(1):105-117,13.基金项目
国家自然科学基金(31972782 （）
32273102)、国家重点研发计划(2018YFD0900103 （）
2018YFD0900404)、中国科学院先导专项(XDA24030105)和财政部和农业农村部:现代农业产业技术体系(CARS-48)共同资助. （XDA24030105）
