中国当代医药2023，Vol.30Issue(36)：22-26,封4,6.
细胞毒性相关辅助T淋巴细胞在精原细胞瘤中的表达及其临床意义
Expression of cytotoxic-associated helper T lymphocytes in seminoma and its clinical significance
韩圣斌 1高宇文 1陈兵华1
作者信息
- 1. 福建省平潭综合实验区医院泌尿外科,福建平潭 350400
- 折叠
摘要
Abstract
Objective To detect the expression of CD4+ cytotoxic-related helper T lymphocytes in normal testis and semi-noma,and to explore their role in the immune microenvironment of seminoma.Methods The gene expression profiling in-teractive analysis(GEPIA)was used to analyze the expression of CD4+ cytotoxic-related helper T lymphocyte marker molecules CD4 and Granzyme K(GZMK)in normal testis and seminoma,and to analyze the correlation of their expression and the relationship between them and tumor stages.The expression levels of CD4,GZMK and CD4+GZMK+in normal testis(n=8)and seminoma(n=12)were detected by immunohistochemistry and double fluorescence staining,and statistical analy-sis was performed.Results The results of GEPIA gene expression profile analysis showed that the expression levels of CD4 and GZMK in seminoma were higher than those in normal testis,and the differences were statistically significant(P＜0.05).The expression of GZMK in high-stage seminoma was lower than that in low-stage seminoma,and the difference was statis-tically significant(P＜0.05).CD4 and GZMK expression levels were positively correlated(r=0.71,P＜0.05).The immuno-histochemical results were consistent with GEPIA gene expression profile data,the expression levels of CD4 and GZMK in seminoma were higher than those in normal testis,and the differences were statistically significant(P＜0.05).Immunofluo-rescence staining showed that CD4 and GZMK expression were co-localized in seminoma.Conclusion The expression lev-els of CD4,GZMK and CD4+GZMK+cells in seminoma tissues were higher than those in normal testicular tissues,suggesting that CD4+cytotoxic T-helper lymphocytes play an important role in the immune microenvironment of seminoma.关键词
精原细胞瘤/CD4+细胞毒性T淋巴细胞/颗粒酶K/肿瘤免疫微环境Key words
Seminoma/CD4+cytotoxic T lymphocytes/Granzyme K/Tumor immune microenvironment分类
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韩圣斌,高宇文,陈兵华..细胞毒性相关辅助T淋巴细胞在精原细胞瘤中的表达及其临床意义[J].中国当代医药,2023,30(36):22-26,封4,6.
