江苏大学学报（自然科学版）2024，Vol.45Issue(1)：112-118,7.DOI:10.3969/j.issn.1671-7775.2024.01.016
一种用于过氧亚硝酸根检测的近红外比率荧光探针的研制
A near-infrared region ratiometric fluorescent probe for peroxynitrite detection
摘要
Abstract
A near-infrared region(NIR)ratiometric fluorescent probe Cy-P based on hemicyanine was rationally designed,prepared and utilized for the detection of exogenous and endogenous peroxynitrite(ONOO-)in living cells.The research results show that Cy-P itself displays NIR emission owing to the large π-conjugated system.With the addition of the strong oxidizer ONOO-,the π-conjugated system of Cy-P is destroyed,and a new substance with blue fluorescence is formed,leading to 268 nm blueshift of fluorescent emission.The linear detection range of the probe Cy-P to ONOO-is0-15 μmol/L,and the detection limit is 13 nmol/L.The probe exhibits superior selectivity for ONOO-over various potentially interfering biological analytes.Due to the low cytotoxicity,the prepared probe is suitable for imaging detection of exogenous and endogenous ONOO-in living cells.关键词
近红外比率荧光探针/过氧亚硝酸根/半花菁/活细胞/荧光成像Key words
NIR ratiometric fluorescent probe/peroxynitrite/hemicyanine/living cells/fluorescence imaging分类
资源环境引用本文复制引用
韩志湘,代晓婷,熊杰..一种用于过氧亚硝酸根检测的近红外比率荧光探针的研制[J].江苏大学学报（自然科学版）,2024,45(1):112-118,7.基金项目
国家自然科学基金资助项目(21105038) （21105038）
江苏高校优势学科建设工程项目(苏政办发[2018]87号) （苏政办发[2018]87号）
苏州科技大学水处理技术与材料协同创新中心资助项目 （）
