军事医学2023，Vol.47Issue(12)：913-918,6.DOI:10.7644/j.issn.1674-9960.2023.12.006
基于内参辅助的新型冠状病毒荧光免疫层析方法
An immunochromatographic method for SARS-CoV-2 based on the assistance of internal reference protein
摘要
Abstract
Objective To monitor the validity of the sample process flow and to improve the detection accuracy of a SARS-CoV-2 antigen by introducing internal reference into SARS-CoV-2 fluorescent lateral flow assay.Methods Silica-core double-layer quantum dots(SiO2-DQD)microspheres were prepared using a polyethyleneimine(PEI)-mediated self-assembly method before being separately conjugated with an internal reference detection antibody and SARS-CoV-2 antibody.Their capture antibodies were plotted on two test lines of the nitrocellulose membrane.Based on double-antibody sandwich detection principles,an internal reference-assisted fluorescent lateral flow assay for SARS-CoV-2 antigen was established.Results According to the readout fluorescent signals,the detection limit of the method for the SARS-CoV-2 antigen reached 0.01 ng/ml with a validated sample process,suggesting its good specificity and stability.Conclusion In this study,a rapid internal reference-integrated fluorescent lateral flow assay for SARS-CoV-2 has been established,which provides control reference for the analysis workflow so that the false-negative rate of the test results can be reduced.关键词
新型冠状病毒/内参蛋白/免疫层析/量子点/荧光Key words
SARS-CoV-2/internal reference protein/immunochromatography/quantum dots/fluorescence分类
基础医学引用本文复制引用
王子仪,程小丹,杨兴胜,荣振,王升启..基于内参辅助的新型冠状病毒荧光免疫层析方法[J].军事医学,2023,47(12):913-918,6.基金项目
国家自然科学基金项目(81902159) （81902159）
