江西农业大学学报2023，Vol.45Issue(6)：1315-1323,9.DOI:10.13836/j.jjau.2023121
水稻SBP基因家族的全基因组研究
Genome-Wide Study of the Rice SBP Gene Family
摘要
Abstract
[Objective]Since the shortage of water resources seriously threatens China's food security,op-timizing the water-saving and drought resistance characteristics of rice is the most economical and effective method to promote water-saving in dryland agriculture.The plant-specific transcription factor squamosa pro-moter binding protein(SBP)affects plant type,yield and stress resistance,which provides important support for water-saving and drought-resistant breeding.However,the functions of SBP family members in rice develop-ment and stress response have not been fully identified,and the regulation of stress resistance has not been stud-ied.[Method]This study utilized SBP domain consistent sequences to search for the rice genome,followed by gene structure and phylogenetic analysis,Co-linear analysis,and gene expression pattern analysis.[Result]19 SBP-LIKE(OsSPL)genes were identified on 10 chromosomes,and the encoding length of amino acid residues ranged from 217 to 1141.Gene structure and phylogenetic analysis suggested that rice OsSPL gene could be di-vided into four groups.Co-linear analysis showed that OsSPL-4 and OsSPL-5 formed a tandem repeat gene cluster within the rice genome,and 11 OsSPLs formed 7 cross chromosomal duplicate gene pairs.5 homologous gene pairs were formed between rice and Arabidopsis SPL,and 45 homologous gene pairs were formed between rice and maize.Furthermore,the analysis of OsSPL gene expression patterns in the Evorepro database showed that under cold stress,the expression of OsSPL-1 and OsSPL-3 in rice leaves was down regulated,while the ex-pression of OsSPL-2 and OsSPL-9 was up regulated;Under drought stress,the transcription levels of OsSPL-1,OsSPL-4 and OsSPL-8 were down-regulated;The transcription levels of OsSPL-2 and OsSPL-14(IPA1)were up-regulated.[Conclusion]The OsSPL gene is widely distributed in the rice genome and forms multiple gene pairs cross chromosome;Under the stresses such as cold and drought,it can make significant responses and thus demonstrate the ability to regulate the stress tolerance and resistance of rice.The results of this study can provide important theoretical support for the improvement and mechanism analysis of water-saving and drought resistant rice(WDR)breeding.关键词
水稻/SBP-box/SPL/基因组共线性/表达分析/抗逆性Key words
rice/SBP-box/SPL/genome synteny/expression analysis/stress resistance分类
农业科技引用本文复制引用
庄可卿,李华,杨婷,宁德正,刘利莎,夏海林,卫海滨,张琳,胡颂平,杜娟,翁雅萍,许雅琴,熊娟,邓志龙,刘昊霖,刘豫,宋惠..水稻SBP基因家族的全基因组研究[J].江西农业大学学报,2023,45(6):1315-1323,9.基金项目
国家自然科学基金项目(31971926)和江西省自然科学基金联合资助重点项目(20202ACBL205007) Project supported by the National Natural Science Foundation of China(31971926)and Jiangxi Provincial Natural Science Foundation Joint Funding Key Project(20202ACBL205007) 江西农业大学2023年大学生创新创业项目(S202310410095、S202310410098、X202310410304)同时对本研究给予了资助,谨致谢意! （31971926）
