江西农业大学学报2023，Vol.45Issue(6)：1324-1330,7.DOI:10.13836/j.jjau.2023122
谷子SiPRR73基因在不同光温组合条件下的可变剪切分析
Alternative Splicing Analysis of SiPRR73 under Different Photo-thermal Combinational Conditions in Foxtail Millet(Setaria italica L.)
摘要
Abstract
[Objective]This study analyzes the alternative splicing variants to explore probable mechanism of SiPRR73 in regulating interaction between photoperiod and temperature in foxtail millet.[Method]The circadian clock gene SiPRR73 was cloned from'Yangu 11'by RT-PCR.The cDNA sequences of SiPRR73 under low temperature(15℃)and natural condition,four photo-thermal combinational conditions were cloned respectively for variable splicing analysis.[Result]The amplified fragments were cloned,sequenced,and spliced to obtain a 2928 bp cDNA sequence,containing a CDS region of 2283 bp and encoding 760 amino acids.Alternative splicing analysis showed that the splicing variant(SiPRR73-1,SiPRR73-3)detected only under low temperature(15℃)and low temperature and long-day(22℃and 15 h light/9 h dark)condition contained complete coding area,and it contained complete REC and CCT domains,the two splicing variants(SiPRR73-2,SiPRR73-4)detected under natural condition and low temperature and short-day(22℃and 9 h light/15 h dark)lacked complete CCT domain.under either long-day or short-day temperature(27℃),two splicing variants(SiPRR73-5,SiPRR73-6)that lacked both REC and CCT domains were detected.[Conclusion]SiPRR73 participated in regulation of interaction between photoperiod and temperature through alternative splicing ways.关键词
谷子/PRR73/光温互作/可变剪切Key words
foxtail millet/PRR73/photo-thermal interaction/alternative splicing分类
农业科技引用本文复制引用
王晓蒙,李兴杰,董志平,贾小平,李剑峰,刘辉,王永芳,张小梅,王振山,桑璐曼,闫留延,龚珂珂..谷子SiPRR73基因在不同光温组合条件下的可变剪切分析[J].江西农业大学学报,2023,45(6):1324-1330,7.基金项目
国家自然科学基金项目(31471569) Project supported by the National Natural Science Foundation of China(31471569) （31471569）
