江西农业大学学报2023，Vol.45Issue(6)：1358-1369,12.DOI:10.13836/j.jjau.2023125
外源ABA处理对红阳猕猴桃果实采后成熟及关键基因的影响
Effects of ABA Treatment on Postharvest Ripening and Key Genes of Actinidia chinensis cv.'Hongyang'
摘要
Abstract
[Objective]The aim of this study was to investigate the effects of abscisic acid(ABA)treatment on postharvest fruit ripening and fruit quality of kiwifruit,thus providing theoretical basis and reference for precise regulation of postharvest ripening process and prolonging storage period of kiwifruit.[Method]In present study,'Hongyang'was used as the experimental material.The kiwifruit clusters were treated with 100 µmol/L ABA and 200 µmol/L nordihydroguaiaretic acid(NDGA,an inhibitor of ABA synthesis)for fruit soaking after harvesting,and water treatment was used as the control.Postharvest fruit respiration intensity,weight loss,hardness,soluble solids,titratable acid,vitamin C and soluble sugar contents were measured to investigate the effects of ABA treatment on the storage quality of kiwifruit fruit.Endogenous ABA content and ethylene release were detected,the expression of key genes of ABA metabolism(AcNCEDs and AcCYP707As)and core component genes of ABA signaling(AcPYLs,AcPP2Cs and AcSnRK2s),as well as the transcriptional expression differences of ethylene-related and cell wall degradation-related genes were analyzed by qRT-PCR.[Result]During the storage period at room temperature,fruit hardness of kiwifruit fruits gradually decreased,and the hardness of all ABA-treated groups were lower than that of the control group,while at the 8th day of storage,ABA significantly increased the expression of cell wall degradation-related genes AcPL1,AcPL2,AcXTH5,AcXTH6,AcPME1 and AcMAN1,which were 3.01,2.42,7.58,21.47,5.11 and 9.49-fold,respectively,indicating that ABA treatment may promote fruit softening by inducing the expression of genes related to cell wall degradation.Exogenous ABA promoted endogenous ABA biosynthesis and ethylene release in kiwifruit.Tthe expression patterns of AcNCED1 and AcNCED2 were consistent with the trend of ABA content,while the expression analysis of ethylene-related genes revealed that ABA treatment promoted the up-regulation of ethylene synthesis and signal transduction genes.In addition,ABA treatment promoted the degradation of titratable acid and vitamin C,the accumulation of soluble solids,total sugars and soluble sugars,and increased the fruit respiration rate and weight loss rate.These results suggest that ABA promoted postharvest ripening of kiwifruit and affected fruit quality.[Conclusion]Exogenous ABA treatment promotes postharvest ripening of kiwifruit fruit,stimulats the expression of ethylene synthesis and signaling-related genes,as well as cell wall degradation-related genes,and accelerates fruit softening.The results provide a basis for revealing the molecular mechanism of ABA-promoted postharvest ripening in kiwifruit.关键词
ABA/猕猴桃/采后成熟/基因表达Key words
ABA/kiwifruit/postharvest/gene expression分类
农业科技引用本文复制引用
兰德国,廖星强,翟夏琬,贾茗淑,赵连鑫,杨清群,陈金印,开文斌..外源ABA处理对红阳猕猴桃果实采后成熟及关键基因的影响[J].江西农业大学学报,2023,45(6):1358-1369,12.基金项目
国家自然科学基金项目(32260780)和中国博士后科学基金项目(2021M701513) Project supported by National Natural Science Foundation of China(32260780)and China Postdoctoral Science Foundation(2021M701513) 江西省研究生创新专项资金项目(YC2022-s430)同时对本研究给予了资助,谨致谢意! （32260780）
