江西农业大学学报2023，Vol.45Issue(6)：1486-1495,10.DOI:10.13836/j.jjau.2023136
饲料缬氨酸添加水平对意大利蜜蜂幼虫生长发育、血液生化指标及相关基因表达量的影响
Effects of Dietary Valine Supplementation Levels on Growth and Development,Blood Biochemical Parameters and Related Gene Expression in Apis mellifera L.Larvae
摘要
Abstract
[Objective]This study aims to determine the effects of dietary valine on the growth,development,hemolymph biochemical parameters,and expression of genes related to the PI3K-AKT-TOR signaling pathway in honeybee larvae(Apis mellifera L.).[Method]A total of 4801-day-old female Italian honeybee larvae were randomly divided into five groups with four replicates of 24 larvae each.The control group was fed a basal diet(0%),and the experimental groups were fed the basal diet supplemented with 0.25%,0.50%,0.75%,and 1.00%valine,respectively.The final larval weight,body composition(moisture content,crude protein),pupation rate,emergence rate,hemolymph amino acids and biochemical parameters,and expression of genes related to the PI3K-AKT-TOR signaling pathways were analyzed and measured.[Result]The results showed that compared with the control group,the crude protein content in the valine-supplemented groups significantly increased.The groups supplemented with 0.25%and 0.50%valine showed significant improvements in larval weight,while the 0.50%valine supplementation level significantly increased pupation rate and emergence rate.Compared with the 0.50%valine supplementation group,the groups supplemented with 0.75%and 1.00%valine showed significant reductions in final larval weight,pupation rate,and emergence rate.Valine addition significantly influenced larval hemolymph bar glutamate,glycine,alanine,valine,methionine,leucine,lysine,and proline content.Hemolymph biochemical analysis results indicated that valine supplementation in the basal diet significantly reduced creatine kinase levels,and high levels of valine supplementation significantly increased aspartate transaminase levels.In addition,the supplementation levels of 0.75%and 1.00%valine significantly downregulated the expression of AmTOR,Amp70S6K,and Am4E-BP.[Conclusion]It can be concluded that appropriate supplementation of valine in the basal diet can promote the growth and development of honeybee larvae and improve their health status.Regression analysis results suggested that the optimal level of valine supplementation in honeybee larval feed under the experimental conditions was 0.3089%.This study revealed the relationship between valine requirement and growth and development of honeybee larvae,providing a theoretical basis for determining the suitable valine level in honeybee larval feed.关键词
意大利蜜蜂/幼虫/缬氨酸/氨基酸代谢/生长发育Key words
Apis mellifera L./larvae/valine/amino acid metabolism/growth and development分类
畜牧业引用本文复制引用
马兰婷,程文艺,王帅,胥保华,王颖..饲料缬氨酸添加水平对意大利蜜蜂幼虫生长发育、血液生化指标及相关基因表达量的影响[J].江西农业大学学报,2023,45(6):1486-1495,10.基金项目
山东省自然科学基金项目(ZR2022MC069)和国家现代农业产业技术体系项目(CARS-44) Project supported by the Natural Science Foundation of Shandong Province(ZR2022MC069),the China Agricul-ture Research System(CARS-44) 泰山产业领军人才高效生态农业创新类项目(LJNY202003)同时对本研究给予了资助,谨致谢意! （ZR2022MC069）
