检验医学与临床2024，Vol.21Issue(1)：24-27,4.DOI:10.3969/j.issn.1672-9455.2024.01.006
甘肃地区乙型肝炎病毒分型和耐药突变分析
Genetyping and drug resistance mutation analysis of hepatitis B virus in Gansu area
摘要
Abstract
Objective To analyze the genotype distribution characteristics and drug resistance situation of hepatitis B virus(HBV)in Gansu area,so as to provide a guidance basis for antiviral medication for the pa-tients with hepatitis B.Methods A total of 131 patients with hepatitis B admitted to this hospital from Janu-ary 2018 to August 2019 were selected.The distribution situation of HBV genotypes and drug-resistant muta-tion sites was analyzed by the Sanger sequencing method.Results Among 131 patients with hepatitis B,the genotype B,C and D were detected out,including 2 cases(1.53％)of type B,126 cases(96.18％)of type C and 3 cases(2.29％)of type D.Fifty-one cases of nucleotide drug resistance were detected with a total drug resistance rate of 38.93％,including 1 case of type B,49 cases of type C and 1 case of type D.M204V/I was the most common drug-resistant mutation site,and the M204V/I+M204V combined mutation was more com-mon in multi-stie mutation.The mutations at three sites,four sites and even five sites often occurred on this basis.Adefovir dipivoxil-related A181V/T,N236T,and A181V/T+N236T single-agent mutations were the most common,which had 15 cases(29.41％).There were 36 cases(70.59％)of multi-drug mutations,which was dominated by lamivudine(LAM)and telbivudine(LDT)combined resistance,on this basis more Ente-cavir(ETV)combined resistance occurred.Conclusion The genotype in the patients with hepatitis B in Gan-su area is mainly the genotype C,the drug resistance situation is severe,and its mutation combination forms are complex and diverse.Therefore,the HBV genotype and drug resistance mutation site should be detected in time to evaluate the clinical efficacy of the patients with hepatitis B and guide the drug rational use in clinical antiviral therapy.关键词
乙型肝炎病毒/Sanger测序/耐药突变/基因型/甘肃Key words
hepatitis B virus/Sanger sequencing/drug-resistant mutation/genotype/Gansu分类
医药卫生引用本文复制引用
丁丽,陈俏丽,田鹏飞,张旭强,雷志萍..甘肃地区乙型肝炎病毒分型和耐药突变分析[J].检验医学与临床,2024,21(1):24-27,4.基金项目
甘肃省兰州市卫生健康指导项目(2022-ZD-20) （2022-ZD-20）
甘肃省兰州市卫生健康行业科研项目(2023-019). （2023-019）
