昆虫学报2023，Vol.66Issue(11)：1443-1450,8.DOI:10.16380/j.kcxb.2023.11.003
基于转录组数据的中华按蚊细胞色素P450超家族基因不同发育阶段、成蚊不同组织和吸血前后不同时期雌成蚊的表达模式分析
Expression profiling of the cytochrome P450 superfamily genes in different developmental stages,tissues and female adults at different periods before and after blood meal of Anopheles sinensis(Diptera:Culicidae)based on the transcriptomic data
摘要
Abstract
[Aim]In this study,the cytochrome P450 superfamily genes of Anopheles sinensis were used to analyze their expression pattern in different developmental stages,different adult tissues and female adult at different periods before and after blood meal of A.sinensis,so as to lay the foundation for further studies on the function of the cytochrome P450 superfamily genes.[Methods]Samples were collected from A.sinensis laboratory deltamethrin-susceptible strain(WX-LS)at different developmental stages(egg,1st-4 th instar larvae,male pupa,female pupa,male adult and female adult),adult tissues(antenna,salivary gland,midgut,Malpighian tubules,testis,ovary,cuticle and fat body),and female adult at 1,3,6,12,24,and 48 h after blood meal to perform transcriptome sequencing using Illumina HiSeq™2000.Based on the sequenced transcriptome data,the expression levels of cytochrome P450 genes in different developmental stages,different adult tissues and female adult before and after blood meal of A.sinensis were analyzed.[Results]The expression of the cytochrome P450 superfamily genes in A.sinensis was both developmental stage and tissue specific.In the larval,pupal and adult stages,40,26 and 35 genes were expressed at high levels,with CYP6AG1,CYP6AG2,CYP6AH1,CYP6Z3,CYP6Y2,CYP6P4,CYP9J3 and CYP9J4 being highly expressed throughout the larval stage.In A.sinensis adult,CYP6AA1,CYP6AH1,CYP6M4,CYP9J3,CYP9J4 and CYP12F3 were highly expressed in the midgut and Malpighian tubules,and CYP4G16,CYP6AA1,CYP6AH1,CYP6Y1,CYP6Z3,CYP9J3 and CYP9J4 were highly expressed in all adult tissues.CYP4G16,CYP6AA1,CYP6AG2,CYP6AH1,CYP6Y1,CYP6Y2,CYP9K1,CYP12F3 and CYP304B1 in female adult of A.sinensis were highly expressed at 1,3,6,12,24 and 48 h after blood meal.[Conclusion]Cytochrome P450 superfamily genes highly expressed in the larval stage may be involved in the detoxification of food-borne toxic chemicals,and those highly expressed in the midgut and Malpighian tubules of female adult may play an important role in the detoxification process.These results lay a preliminary foundation for further investigation of the functions of cytochrome P450 superfamily genes in the growth and development,expression regulation and insecticide metabolism mechanism of A.sinensis.关键词
中华按蚊/细胞色素P450/表达模式/发育阶段特异性/组织特异性Key words
Anopheles sinensis/cytochrome P450/expression pattern/developmental stage specific/tissue specific分类
生物科学引用本文复制引用
郭迎澳,陈斌..基于转录组数据的中华按蚊细胞色素P450超家族基因不同发育阶段、成蚊不同组织和吸血前后不同时期雌成蚊的表达模式分析[J].昆虫学报,2023,66(11):1443-1450,8.基金项目
国家自然科学基金项目(31672363,31872262) （31672363,31872262）
国家科技基础性工作专项重点项目(2015FY210300) （2015FY210300）
