昆虫学报2023，Vol.66Issue(11)：1482-1489,8.DOI:10.16380/j.kcxb.2023.11.007
亚洲玉米螟对球孢白僵菌分生孢子和芽生孢子-玉米共生体的取食选择和嗅觉反应
Feeding preference and olfactory responses of Ostrinia furnacalis(Lepidoptera:Pyralidae)to Beauveria bassiana aerial conidia and blastospore-corn symbionts
摘要
Abstract
[Aim]Beauveria bassiana can indirectly affect phytophagous insect pests by colonizing plants.This study aims to determine the responses of feeding preference and olfactory response of the neonate and 3rd instar larvae of Ostrinia furnacalis to symbionts of aerial conidia(AC)and blastospore(BS)B.bassiana-corn..[Methods]Each com plant was inoculated with 20 mL B.bassiana spore suspension(1.0 × 108 spore/mL),soil drench inoculation was used to establish the B.bassiana-corn symbionts,including the B.bassiana AC treatment group and the B.bassiana BS treatment group.The feeding selection rates by the neonate larvae and 3rd instar larvae of O.furnacalis to the corn leaves of the B.bassiana-corn symbionts of AC and BS treatment groups under the choice and non-choice conditions were tested by a dish leaf disc method.The olfactory selection coefficient and olfactory selection response rate of the neonate and 3rd instar larvae of O.furnacalis to the corn leaves of the B.bassiana-corn symbionts of AC and BS treatment groups were detected by Y-type olfactometer.[Results]The test results of the dish leaf disc method showed that the feeding selection rate of both the neonate and 3rd instar larvae of O.furnacalis to the corn leaves of the B.bassiana-corn symbionts of AC and BS treatment groups were lower than that of the control group under the choice and non-choice conditions.The neonate larvae of O.furnacalis had the lowest feeding selection rate to the corn leaves of the B.bassiana-corn symbionts of BS treatment group under the choice condition,while the neonate larvae of O.furnacalis had no significant difference in the feeding selection rate to the corn leaves of the B.bassiana-corn symbionts of AC and BS treatment groups under the non-choice condition.The results of Y-type olfactometer test showed that the olfactory selection response rate of both the neonate and 3rd instar larvae of O.furnacalis to the corn leaves of the B.bassiana-corn symbionts in AC and BS treatment groups(olfactory selection coefficient＜0)were significantly lower than that in the control group(olfactory selection coefficient＞0),suggesting that the two types of B.bassiana spore colonized corn leaves have negative chemotaxis to O.furnacalis larvae.[Conclusion]This study confirms that there is an obvious avoidance effect of the corn leaves colonized by aerial conidia and blastospores of B.bassiana on the feeding behavior and olfactory behavior of O.furnacalis,which could provide a theoretical basis to utilize the endophytic colonization of B.bassiana for ecological control of insect pests.关键词
亚洲玉米螟/虫生真菌/植物内生性/行为反应/叶碟法/Y型嗅觉仪Key words
Ostrinia furnacalis/entomopathogenic fungi/plant endogeneity/behavioral response/leaf disc method/Y-type olfactometer分类
生物科学引用本文复制引用
隋丽,路杨,谢敏,邹晓威,李启云,张正坤..亚洲玉米螟对球孢白僵菌分生孢子和芽生孢子-玉米共生体的取食选择和嗅觉反应[J].昆虫学报,2023,66(11):1482-1489,8.基金项目
国家自然科学基金项目(32271683) （32271683）
吉林省自然科学基金项目(20220101313 JC) （20220101313 JC）
吉林省科技厅中青年科技创新创业卓越人才(团队)项目(创新类)(20230508011RC) （团队）
吉林省科技发展计划中青年科技创新创业卓越人才(团队)项目(20210509014RQ) （团队）
