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实验教学中醋酸纤维素薄膜电泳的条件优化

武丽涛 李梦瑶 宁启兰 吴锋 杨旭东 闫小飞 蒋小英 蒋晓刚 李冬民

南阳师范学院学报2024,Vol.23Issue(1):74-78,5.
南阳师范学院学报2024,Vol.23Issue(1):74-78,5.

实验教学中醋酸纤维素薄膜电泳的条件优化

Optimization of conditions for cellulose acetate membrane electrophoresis in experimental teaching

武丽涛 1李梦瑶 1宁启兰 1吴锋 2杨旭东 1闫小飞 1蒋小英 1蒋晓刚 1李冬民1

作者信息

  • 1. 西安交通大学 医学部基础医学院生物化学与分子生物学系,陕西 西安 710061
  • 2. 西安交通大学 医学研究生教学实验中心,陕西 西安,710061
  • 折叠

摘要

Abstract

Separation of serum proteins by cellulose acetate membrane electrophoresis is a basic experimental technique in biochemistry laboratory classes.At present,many medical schools offer this experiment in basic medicine and biology.However,due to different experimental methods and many influencing factors in the ex-perimental process,the experimental results obtained by students have various problems.After years of experi-mental teaching exploration,the experimental protocol for the separation of serum proteins by cellulose acetate membrane electrophoresis has been optimized,the interactivity of the experimental teaching content has been en-hanced,and the stability of the experimental results has been ensured,which enable the students to better grasp the theoretical knowledge of the horizontal electrophoretic separation of proteins and obtain a successful experi-mental verification.

关键词

生物化学实验教学/醋酸纤维素薄膜电泳/血清蛋白的分离

Key words

laboratory teaching of biochemistry/cellulose acetate membrane electrophoresis/separation of ser-um proteins

分类

生物科学

引用本文复制引用

武丽涛,李梦瑶,宁启兰,吴锋,杨旭东,闫小飞,蒋小英,蒋晓刚,李冬民..实验教学中醋酸纤维素薄膜电泳的条件优化[J].南阳师范学院学报,2024,23(1):74-78,5.

基金项目

西安交通大学"名课程"《生物化学》第三轮建设立项课程 ()

2021 年西安交通大学本科实验实践与创新创业教育教学改革研究专项项目(21SJZX29) (21SJZX29)

南阳师范学院学报

OACHSSCD

1671-6132

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